A Preliminary Study On Toxicity Control Of Acrylamide

Acrylamide produced in heat-processed foods had been known to be neurotoxic,genotoxic and carcinogenic in animals.Presently, investigation of inhibiting acrylamide-induced toxicity had triggered worldwide concern.It was well known that acrylamide converted into glycidamide was catalyzed from P450 2E1 in vivo,which played an important role in toxicity effects.Exogenous antioxidant can inhibit acrylamide toxicity bycontrolling epoxidation to decrease the process of its conversion into glycidamide such as Vitamine C,Methionine,Allicin, etc.There was a preliminary study on reducing acrylamide toxicity by controlling epoxidation and elimination of acrylamide.1.Maillard reaction in foods produced acrylamide and other products which exhibited antioxidant activity.The aim of this study was to estimate the correlation of acrylamide with antioxidant activity of Maillard reaction products(MRPs)by a glucose-asparagine model.Acrylamide in reaction solutions was determined by reversed phase high performance liquid chromatography(RP-HPLC).Antioxidant activity was characterized by 1,1-dipheny-2-picrylhydrazyl(DPPH), reducing power and hydroxyl radical scavenging activity.Factors including pH,temperature,reaction time,the volume ratio of glucose-to-asparagines,the sorts of amino acid as well as sugar affecting the acrylamide formation were tested with glucose-asparagines as a model.By the way,products from Maillard reaction exhibited antioxidant activity.The result in this paper showed the more concentration of acrylamide,the higher antioxidant activity of MRPs(hydroxyl radical scavenging activity presented good correlation with acrylamide treated between 120℃and 180℃).Antioxidant activity of MRPs may inhibit acrylamide toxicity by preventing the process of epoxidation from P450 2E1 to decrease glycidamide.2.Acrylamide-glutathione reaction simulated in vitro was investigated.The reaction products were directly excreted in urine,which decreased the absorption of acrylamide not to be converted into glycidamide.Reducing of acrylamide absorption will provide a new way for controlling acrylamide toxicity.A RP-HPLC method was developed for determination of glutathione sulfhydryl to observe the acrylamide-glutathione reaction and pseudo-first-order rate constants and second-order rate constants of acrylamide with glutathione were 0.0491 min^-1and 0.0001 mM^-1min^-1 respectively.It was showed that the process of reaction will decrease the concentration of acrylamide for controlling acrylamide toxicity.