Study On Extraction And Antioxidant Of Polyphenol From Molasses Alcohol Wastewater

Alcohol wastewater is a high-concentration organic wastewater with pigments.The colour of pigments is very deep,very hardly degradned,and it is the important reason that COD and BOD is so high in alcohol wastewater.The pigment from molasses alcohol wastewater is a natural pigment,which is formed and accumulated in the sugar growing and processing.It includes polyphenol pigment,caramel pigment and maillard pigment.It is reported that polyphenol pigment has the antioxidant activity and is used as a nature antioxidant,which is used generally much vocation(Food,Medicine,Cosmetics).So it is important to extract polyphenol pigment from alcohol wastewater.It is advantageous for us to solve pollution problem when attain the antioxidant with high additional value.The main research contents and results of this thesis are reported as following: 1.The extracting result of alcohol was studied on powdery pigment from alcohol wastewater.By the one factor and orthogonal experiment, the best extracting condition is:95%aqueous ethanol,temperature 50℃, ratio of liquid/solid as 1:15,time 1h,degree 3.2.The samples were separated and purified with membrane and polyamide.By membrabe experiment,it was found that 300D～800D group had strongest antioxidant activity.By polyamide experiment the best purification condition is:temperature 40℃,adsorption time 30min, 60～80 orders polyamide,90%ethnol solution desorption,the large adsorption capacity was 90.5mg per polyamide.3.The antioxidant activity of samples was studied by four different system(oil,·OH,DPPH·and linoleic acid).The result showed that samples had certain antioxidant activity.In all system the antioxidant activity of the sample A was less power than the sample B.Their antioxidant activity enhanced with adding of concentration.4.The detection of UV showed that samples contained flavonoids. The detection of IR showed that samples contained hydroxy,phenyl,carbonyl and had typical character spectrum of phenol compound.The detection of HPLC showed that samples contained different matters.Sample B1 contained galic acid,rutin,chlorogenic acid,while sample B2 contained galic acid,quercetin,catechin and chlorogenic acid.Sample B1 contained more flavone,while sample B2 contained more flavonid glycoside.