| Polyphenol and phytic acid have a more extensive utility in the food industry and other areas. Many literatures about the plant material in the production of the phytic acid and polyphenol had been reported. However, polyphenol and phytic acid are the main anti-nutritional substance in the rapeseed meal. Double low rapeseed had become the bottleneck in the comprehensive utility of rapeseed meal. Therefore, effective extraction and preparation polyphenol and phytic acid from rapeseed meal and enhancement of its bio-activity become the key point in the process of rapeseed meal.In this study, while rapeseed meal in the extraction of polyphenol and phytic acid for innovation, compared with the former extraction step by step, simplified the extraction process, might save the partial process unit for the rapeseed cake deep-processing, and the results of extraction meet the expected demand.In this study, Hubei Xishui Zhayou plant providing the "double low" hybrid strain.Cold pressing meal on the No.4 rapeseed meal for the experiment.The pretreatment immersed with acid ethanol and added a small amount of additives of dodecyl benzene sulphonic acid, and then extracted the phytic acid and polyphenol from rapeseed meal at the same time, and use the response surface to analysis and determine optimum conditions. Using solvent of sodium hydroxide to precipitate the phytic acid, then it was purified tthrough ion exchange resins. After the treatment mentioned above, the phytic acid samples was analyzed. The polyphenol,in the precipitation solvent, was extracted by using of organic solvent, and then it was purified by macroporous resin to classify to several grades.The level of different sub-components were analyzed. The results are as follows:1. The optimun extraction condition of extracting the phytic acid and polyphenol at the same: The acid ethnol with the additive of dodecyl benzene sulphonic acid was the extraction solvent.Through the single-factor analysis and response surface the optimun extraction conditions was determined: 80 mesh rapeseed cakes, 12.50:1 fluids materials ratio, 54℃, 57minutes, two times extraction, respectively. In this condition, the yields of polyphenol and phytic acid were 2.64% and 4.86%, respectively.2. Purification and characterization of the phytic acid: The phytic acid were precipitated by NaOH soultion. Dissolved by hydrochloric acid, sample concentration was turned to about 4.71mg/ml. Firstly the sample was purified with ion exchange resins D315 as rate of flow 1ml/min, then it was decompressed enriched. Finally it tackled with the processing line of Sodium phytate, getting the production of Sodium phytate with 84.2%. Scaned by UV spectrometer, the phytic acid has maxmum wavelength in 260nm, same with phytic acid standard's absorption peak.Compared to standard's HPLC chramatogram, the sample had same retent peak about 4min. so did the infrared map. So it proved they were the same compound.3. purification and characterization of polyphenol: The concentration of crude polyphenol rised by 9.61% through the treatment of petroleum ether and chloroform. Different molecule weight of polyphenol had different polarity. Differet polyphenol with different distribution of molecule weight could been seperated through extraction solvent of ethyl acetate, water, methanol. Raw material could adsorped by macroporous resin. And then the polyphenol was rinsed by 60% ethnol.Compared with polyphenol standard sample, its concentration was as high as 65.48%. Scaned by UV spectrometer, the polyphenol has maxmum wavelength in 280nm, as the tannin. The sample was detected through RP-HPLC, while mobile phase was 30% acetonitrile. the results showed that ethyl acetate grade has earlier retent time thanother grade, aqueous phase secondly and methanol phase thirdly.
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