Influence Of Escherichia Coli ER2566 By Black Soldier Fly Larvae Grown In Dairy Manure

With the development of confined dairy facilities,dairy manure waste can also be expected to expand,consequently,dairy manure waste becomes a huge pollution source of agriculture and poses a potential threat to environment without proper disposal.Dr.Jeffery Tomerlin (USA),has been succeed in managing livestock manure from confined dairy facilities using black soldier fly,Hermetia illucens L.,and black soldier fly could be proposed as a new method to solving environmental pollution.On the basic of his study,the study focus on influence of Escherichia coli ER2566 by black soldier fly larvae grown in dairy manure and the treatment of black soldier fly(BSF) digested dairy manure.In the first part,in order to determined if black soldier fly could accelerate the inactivation of Eschefichia coli in dairy manure,we examined the relationship of manure density, temperature,storage time to the inactivation of E.coli,and also monitored the growth of E. coli,larvae and moisture loss of dairy manure,finally proved the hypothesis and the optimization of conditions from assessment of above aspects.The results can be seen as follows:black soldier fly larvae could reduce green fluorescent protein-labeled Escherichia coli ER2566(ER2566-pQBI63) loads in dairy manure,and we examined influence of manure density,temperature,storage time on the inactivation of ER2566-pQBI63,figured out the optimization of conditions for inactivation of ER2566-pQBI63 by black soldier fly larvae:ten grams of 15-day old larvae and ER2566-pQBI63(about 10~7 CFU/g,the ratios of ER2566-pQBI63 and manure was 1:100) were incubated into 50 g of autoclaved dairy manure and held at 27℃for 72 h in growth chamber,the populations ER2566-pQBI63 were not detected.As a result,it indicated that black solider fly larvae can be served a new effective method to control pathogens in dairy manure and guided to research of antimicrobial effect of black soldier fly larvae.In the second part,the study was to compare the use of black soldier fly(BSF) digested dairy manure to LG3 Mix(a commercial greenhouse potting mix) for the growth of three kinds of forages(annual ryegrass,Lolium multiforum;wheat,Triticum aestivum;hairy vetch,Vicia villosa) under greenhouse conditions,and measured seed germination ratio,biomass dry matter,and nutrition value(protein concentration) to figure out if BSF digested dairy manure was an alternative for LG3 Mix used for potting systems,and suitable for some kind of forage.The results can be seen as follows:BSF digested dairy manure was an alternative for LG3 Mix used for potting systems,and suitable for annual ryegrass and wheat.The results shown that annual ryegrass and wheat grown in BSF digested dairy manure differed significantly from those grown in LG3 Mix with respects to seed germination ratio,above& below-ground biomass and total biomass dry matter yields,crude protein concentration of above-ground and total biomass:(1) seed germination ratio were 100%,100%,respectively; (2) above-ground biomass dry matter were 10.8g/stock,11.1 g/stock,and increased by 100%,82.3%,respectively;(3) below-ground biomass dry matter were 2.02 g/stock,1.94 g/stock,and increased by 106%,105%,respectively;(4) total biomass dry matter were 13.3 g/stock,12.9 g/stock,and increased by 109%,86.7%,respectively;(5) crude protein concentration of above-pound biomass were 2.50 g/stock,2.77 g/stock,and increased by 67.9%,44.7%;(6) crude protein concentration of total biomass 2.70g/stock,2.94 g/stock,and increased by 69.8%,45.5%,respectively.While hairy vetch did not have any significant difference,except the seed germination ratio was as low as 90%.As a result,it shown that BSF digested dairy manure was used as a fertilizer,it will not only be proposed as a means to manage dairy wastes,but also severed as a new renewable natural resource.