Study On Extraction, Purification And Antioxidant Of Anthocyanins From Three Wild Berry

Anthocyanin is foliage polyphenol compound.They are glycosides of polyhydroxy and polymethoxy derivatives of 2-phenylbenzopyrylium or flavylium salts.Anthocyanins are naturally occurring compounds that impart color to plants,fruits,and vegetables.Nowdays many anthocyaninss of man-made used in the food industry,and all of them had dissimilar grade toxicity,it would endanger the human health during the long time using,so the nature anthocyanins had arosed more attention from the scientists.Anthocyanins not only had the good anthocyaninsation,but also had an array of health-promoting benefits,as they could protect against a variety of oxidants,inhibit the growth of human tumor cells,prevent the vasal disease of heart and brain.This article mainly studied the anthocyanin on extraction, purification and antioxidant activity,based on the three northeast material,such as wild berry of Lonicera edulis Turcz,Glycine max(L.) Merr.,Vaccinium uliginosum L..This thesis adopted the method of acidic ethanol and ultrasonic to extract the anthocyanins. The optimal process in extractive method with acidic ethanol was ascertained by the single factor experiment and the Response Surface Methodology(RSM).The optimal extraction conditions were that the concentration of ethanol was 69.8%,the extractive time was 2.56h, extractive temperature was 55.1℃,and the ratio of materiel to liquid was 1:20,the extractive times was 2,and after twice,the extraction ratio was 93.0%.The optimal process on extractive method by ultrasonic was ascertained by the single factor experiment and the orthogonal design.The optimal extraction conditions were that the ultrasonic response time was 30min, the ultrasonic power was 500W,and the ratio of materiel to liquid was 1:15.Studying the influence of temperature,pH,light,metal ion and several normal food additive to threeanthocyanins.Results showed that,the three anthocyanins had well stability when the temperature below 60℃,when the temperature at 100℃,the sample was quickly destroyed; the three anthocyanins was sensitive to pH value,Vc,light;some mental ions such as Al³⁺,and some food additive such as NaCl,sugar,citric acid made the anthocyanins deepen its color and raise its stability,but others such as Fe³⁺ made it fade and decreased its stability.The anthocyanin was purified by macroporous adsorption resin in this thesis.Compared with eight kinds of resin,the best one was X-5,and ethanol had the best desorption.Result of static state showed,X-5 resin was the model of fast balance,and the concentration of ethanol was 60%,the time of balance was 140min,the best pH value was 2.0.Result of dynamic state showd:the optimal purification conditions were extract sample velocity 1.0 ml/min,extract sample concentration 8g/L,eluting ethanol velocity 1.0 ml/min.The X-5 absorption capacity of saturation was 0.0517g/ml.After eight times,the ratio of adsorption did not change,and it meaned that it had good capability of circular using.The antioxidation of anthocyanin which purified preliminarily was determined using total antioxidative activity,hydroxyl free radicals,superoxide anion free radicals,DPPH free radicals,reducing force,lipidosome antioxidation reaction system.Results showed that all of the three samples had strong antioxidative activity,and as increasing the concentration,the antioxidative activity being stronger.In the total antioxidative activity and hydroxyl free radicals,the three samples had stronger antioxidative activity than any other reaction system...