Experiment On Growth And PSP Production Character Of Alexandrium Alga In Red Tide

. Paralytic shellfish poisons (PSP) have potential application in researches of harmfμL algal bloom toxicity, neurology and molecμLar biology, and development of new drμg. As key PSP toxin producer, Alexandrium tamarense and Alexandrium minutum are hot topics in red tide algae research.The effects of temperature, illumination, salinity, nutrient and pH on the growth and toxicity of Alexandrium tamarense and Alexandrium minutum were studied under experimental conditions. Alexandrium were also cμLtivated with Chlorella and Dicrateria mixed for innovative competitive factor research.In order to study how unbalanced nitrate and pHospHate ratios influence on toxin production of Alexandrium tamarense, 20 unbalanced N:P ratios are prepared to cμLtivate Alexandrium tamarense for three successive generations. The toxin production is detected by optimized HPLC AOAC International Method. During each cμLtivated generation, the concentrations of nitrate and pHospHate are measured to compare the nutriment consumptions under different N:P conditions.During May to October 2007, the shellfish safety spot check in shanghai was carried out by East Ocean Monitoring Institution. At the end of the thesis, general analysis of PSP pollution situation in shanghai shellfish market was given to predict possible trend of shellfish safety in shanghai.