The Preparation Of Microbial Oil-based Lubricant

The microbial oil was gotten by fermentation,oxidation reaction was used to modify the oil to change its stability,which can make the oil be a base oil of the lubricant.Then a new type of lubricant was preparated after Schiff base and Schiff base copper complexes were synthesized and dispersed in the base oil with the microemulsion reactor.A rapid identifying of the oil composition and content in microorganisms was established after microwave pretreating, and gas chromatography (GC) was employed to analysis.Only a small amount of wet cell was treated by microwave and reacted directly with KOH-CH3OH,and the reaction product,which is fatty acid methyl ester,was detected by gas chromatography analysis to ascertain the fat content and fatty acid composition in microorganisms.One kind of oil-producing microorganism called Mortierella isabellina CGMCC 3.3410 which has better performance was selected out of six by this method and TEM analysis.Also the characteristics of growth and oil-producing of the Mortierella isabellina CGMCC 3.3410 was studied,and it was cultivated expandedly,then the microbial oil was extracted and purificated.In addition,one oil-producing bacteria was selected from the waste water of a oil factory as one choice of oil-producing microorganisms.It was identified as Mycobacterium,and the oil composition and content under different conditions of culture was also analyzed.The results showed that the rapid identifying method had good precision,accuracy and reliability.The microwave pretreating can improve the oil extraction rate significantly by GC,and SEM analysis also showed the effect of the icrowave.The wet cells can be treated best by 600W power microwave for 2 minutes and sterified with KOH-CH3OH for 2 hours.Besides,a good linear relationship between the content of the oil and the weight of cells can be found while the weight of the wet cells is from 0.1g to 1.0g.The test showed that the iodine value of the microbial oil was reduced significantly and the stability of the lubricant base oil was improved markedly by modification of oxidation.It was confirmed by SEM analyses that,salicylaldehyde-ethylenediamine Schiff base and Cu (II) chelate of bissalicylaldehyde-ethylenediamine as the additive synthesized in the base oil,was dispersed stably by W/O microemulsion reactor including base oil,ethanol and emulsifier.The worn surface of top steel ball after four ball test was analyzed by AES,and it was showed that steel/steel rubbing pairs went through selective transferring processes with such lubricant . It was suggested that the mechanism of the improvement of tribological characteristics of this kind of lubricant was selective transferring effect to wear the surface of self-repair.