Research On Extraction, Segregation And Bioactivity Of Lotus Bee Pollen Polysaccharide

Bee pollen is one of the natural health products, and is known as the almighty nutrition. Polysaccharide is one of the most important components in Bee Pollen, and it is valuable to develop. In this paper, extraction, separation, purification and biological activity of Lotus bee pollen polysaccharide are researched, the results are as follows:1. We used ultrasound-assisted technology in order to extract polysaccharide from Lotus bee pollen. On the basis of single-factors experiments, the Box-Behnken center-united experimental design principles were used to design the experiments and the responsive surfaces analysis of 3 factors and 3 levels was adopted. The quadratic regression equation for rate of polysaccharides was established, and the optimum combination was obtained by responsive surfaces analysis. Experimental results indicated that the ration of solid-liquid,the extraction time and the extraction temperature significantly affected the yield of lotus bee pollen polysaccharide. When the extraction parameters were ration of solid-liquid 1:9.4, 2. 4 hours and 81. 6℃, the ratio of lotus bee pollen polysaccharides was maximum. In the condition, the estimated value was 1. 2201% and verified value was 1. 2317%.2. The studies on effects of different volume ethanol on precipitating Lotus bee pollen polysaccharide showed that, when the volume ratio of ethanol and polysaccharide liquid was 3.5 : 1, we can obtain the maximum polysaccharide. Effects of five different methods on precipitating protein of polysaccharide liquid were studied. The results showed that, the Trypsin+Sevage removal of protein was an effective method and the removal rate of protein reached 82.51%. We found two polysaccharide peaks though Sephadex G-75 elution, and the content of high molecular weight was less than low molecular weight.3. Based on Fenton reaction system,the studies on scavenging effects to hydroxyl radicals of Lotus bee pollen polysaccharide showed that, when the concentration of refined and crude Lotus bee pollen polysaccharide was 2.5mg/mL,the rate of scavenging hydroxyl radical respectively reached 64.58% and 56.82%; Using the pyrogallol autoxidation method, the studies on scavenging effects to superoxide radicals of Lotus bee pollen polysaccharide showed that,when the concentration of refined and crude Lotus bee pollen polysaccharide was 1.3mg/mL, the rate of scavenging superoxide radicals respectively reached 58.28% and 52.52%. So we can conclude that both crude and refined Lotus bee pollen polysaccharide were effective in scavenging superoxide radical and hydroxyl radical.The scavenging capacity for superoxide radical and hydroxyl radical of refined Lotus bee pollen polysaccharide was higher than that of crude Lotus bee pollen polysaccharide.4. The studies on effects of Lotus bee pollen polysaccharide on Hypolipidemic effect of mice showed that, when the Lotus bee pollen polysaccharide was fed to the Hyperlipidemia mice at different dosages which are 150 mg/kg.d, 300 mg/kg.d and 600 mg/kg.d, the effect of Lotus bee pollen polysaccharide on decreasing the content of serum TC in mice was significant,but not on TG and HDL–C. Therefore, the Lotus bee pollen polysaccharide has the Hypolipidemic effect on Hyperlipidemia mice.