A Study On The Extraction And Microcapsule Of The Amur Grape Seed Oil

This research takes the Amur grape seed as raw material. The various factors that had effects on the extraction efficiency of the grape seed oil were systematically studied with supercritical carbon dioxide fluid extraction (SCF-CO2) technology. Oil from Amur grape seeds with two methods of SCF-CO2 and refining after organic solvent extraction was extracted. The physical and chemical index and the nourishment composition of Amur grape seeds oil were mensurated. The method of preparing Amur grape seed oil microcapsule by spray-drying was confirmed. The characters of microcapsuled Amur grape seed oil prepared by spray drying technique was valued, and its period of validity was predicted. The results showed that:1. Extraction of oil from Amur grape seeds with supercritical CO₂ was studied. The results showed that the optimal comminution granularity, pressure, temperature and extraction time were 60, 30 Mpa, 35℃and 160min. On this condition, the production rate of the oil from Amur grape seeds can reach 18.31%.2. Extraction of oil from Amur grape seeds with organic solvent was studied. Through experiment, the optimal solvent, comminution granularity, temperature, extraction time and the proportionment of raw material and solvent were petroleum aether, 60 mu. 95℃. 3 h and 1 : 7. On this condition, the production rate of the raw oil from Amur grape seeds can reach 21.6%. The rate of refined oil was 87.3%.3. Oil from Amur grape seeds with two methods of SCF-CO₂ and refining after organic solvent extraction was extracted. The physical and chemical index and the nourishment composition of Amur grape seeds oil were mensurated. The results showed that oil by SCF-CO₂ extraction excelled the refined oil in main index. The Amur grape seed oil is a kind edible oil of high quality.4. The optimum condition of the emulsifying technique were obtained in the preparation of Amur grape seeds oil microcapsules by spray-drying. The result was as follows: the ratio of glycerol monoester to sucrose fatty acid ester was 1 : 9, the temperature of emulsifying was 80℃, the content of emulsifier was 0.75%. the concentration of emulsion was 20%. the ratio of arabin to maltodextrane was 1: 5, the ratio of oil to wall was 0.5.The optimum spray dehydration technological parameters were confirmed. The result of the orthogenesis test indicates that the optimum parameters about microencapsulated Amur grape seeds oil techniques were as follows: the feed temperature was about 50℃～⁶0℃, the homogenizing pressure was 40 MPa, the temperatures of inlet wind and outlet wind were 180℃and 80℃respectively, the spray pressure was 180 KPa. The microencapsulating efficiency of Amur grape seeds oil was about 77.36% on the condition above.5. The characters of microcapsuled Amur grape seeds oil prepared by spray drying technique was valued. The preparation of Amur grape oil showed milkiness in colour. The density, the content of moisture and solubility were about 0.7312 g/cm³, 2.76% and 94.0%. It provided with certain dispersion and admixture.6. Through experiment, the preserved stability of microcapsuled Amur grape seeds oil excelled other production. The storage life of three kind of production were 140, 102, 46 days, respectively.