| The inulin is the natural levan which withdraws from the medicinal and edible plant Jerusalem artichoke. Because of its good health and medicinal value, inulin has become an internationally popular functional food. In recent years, the Chinese inulin market is also growing. But the anti-oxidation activity, acute toxicity and finished applications about Jerusalem artichoke inulin are rarely reported.The paper took the Jerusalem artichoke tuber as a raw material, with the research on current situation of inulin, combined with the specific experimental conditions, the qualitative and quantitative analysis, extraction and purification process, anti-oxidation activity, acute toxicity of inulin as well as the production of high fruit syrup by inulin hydrolisis were all systematic studied, and one kind of inulin compound product had been initially developed. That would lay the foundation for the future Jerusalem artichoke resources'utilization and development, and create the desired social benefits and its economic value. The major findings were as follows:1. Using spectrophotometry to analyse the inulin content. Total carbohydrate was determined by the method of phenol-sulfuric acid, reducing sugar was determined by the method of 3,5-dinitrosalicylic acid. The method was accurate, repeatable, simple and rapid.2. Used the hot water to extract inulin. The optimal conditions were determined through single-factor test, orthogonal test and analysis of variance:the ratio of sample to water was 1:30, temperature 90℃, time 50 min and the times of extraction was 2. Under this condition, the extract ratio was 78.36%. Strengthening to process 10min by ultrasound in advance, inulin yield could reach to 85.48%. Strengthening to process 4min by microwave in advance, inulin yield could reach to 86.21%.3. The method of calcium hydroxide-phosphoric acid was used to eliminate mixed. The optimal conditions were determined through orthogonal test and analysis of variance:using Ca (OH)2 to adjust pH to 10, using phosphoric acid to adjust pH to 6 and clarify temperature was 80℃.Under this condition, transmittance was 86.0%, the yield of inulin was 72.58%.4. Studied the effects of different resins and activated carbon for decolorization of extract. The results showed that the decolorization effect of D301-G resin was good. The optimal conditions were:inulin concentration 0.20g/mL, feeding temperature 30℃feeding rate 2BV/h, resin consumption 10g/50mL. Used with 732 resin together, decolorization rate was 69.8%, inulin retention rate was 95.2%, transmittance was 93%.5. Studied the preparation of high fructose syrup from inulin with the treatment of acid hydrolysis. The optimum preparation process was:phosphoric acid ratio 2%, temperature 80℃and time 40min. Under this condition, reducing sugar content could reach to 67.83%.6. Inulin sample was identified as homogeneous component by gel filtration, and the purity was high. The monosaccharide composition only included the fructose and the little glucose, and it had the general characteristic of inulin class material.7. Two kind of free radical systems were used to study the antioxidation of inulin and crude inulin. The result indicated that they all had different capability to remove hydroxyl free radicals and superoxide negion, which improved with the increase of inulin concentration. The acute toxicity experiment showed that the daily oral dose of inulin recommended was safe.8.The optimum formula of Inulin compound drink was determined as follows:The ratio of extracts about the chrysanthemum to the Chinese hawthorn was 3:2, Honey 4%, sugar 3%, Inulin2%, CMC-Na 0.1%and alginate 0.05%.
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