Study On The Iridoid Glycosides Preparationan And Serum Pharmacochemistry Of Rehmannia Glutinosa Libosch

Rehmannia glutinosa libosch was originally recorded in"Shennong bencaojing"and classied as the upper class. It is one of the Four famous herbs produced in Henan HuaiQing. It has been used for medical treatment for several thousands years. It has been cultivated in many provinces, but mainly in Wuzhi, Xiuwu and Wenxian and other areas of Henan province. Radix Rehmannia .is the fresh or dry block root or processed product of the Scrophulariaceae plant Rehmannia glutinosa libosch, medicine followed by: fresh Radix Rehmannia, dry Radix Rehmannia and Radix Rehmannia Praeparata. There are distinct differences between the three: fresh Radix Rehmannia can removing heat to promote salivation, cooling blood, and stop bleeding ; dry Radix Rehmannia can removing heat to cool blood,nourishing Yin,body fluid regeneration;Radix Rehmannia Praeparata can nourishing Yin and blood,tonifying didney jing and filling pith. In the Pharmacopoeia of People's Republic of China (2005 edition),which recorded Chinese medicinal formulae, there were one fifth of it concerned Radix Rehmannia.In recent years,researching chemical composition of Radix Rehmannia were common among domestic and oversea scholars. Studies on the constituents of its few real active ingredients, its determination of"active ingredients"are mostly some sort of drugs or indicators contained in the main component composition, there is no basis to prove that as an active ingredients, which also limits the Chinese crude drug developed and utilized.Any drug interactions are related to the human body after the therapeutic work. Shinichi Tashiro Japanese scholar in 1989 proposed the concept of"Serum Medicinal Chemistry"and"Serum Pharmacology".By analyzing the serum after oral administration to determine the composition of traditional Chinese drug directly into the body of material, it become fast, simple and accurate study of the material basis of drug efficacy effective way.In the first chapter,we summarize the study on the chemical,extraction,pharmacological, serum pharmacochemisty of Rehmannia glutinosa Libosch and serum pharmacochemisty of traditional chinese drug.In the second chapter,we extraction Iridoid glycosides from Rehmannia glutinosa Libosch .The materia medica by crushing ,ethanol extraction , Separation and purification through the macroporous resin, silica gel column chromatography, Sephadex column,identify the structure of compound through TLC and spectroanalysis.From the ethanol extract of Rehmannia glutinosa Libosch isolated three compounds .There are Catalpol, Rehmannia glycoside D, Motherwort glycosidesIn the third chapter,we establish experimental method for Serum pharmacochemisty of Rehmannia glutinosa Libosch. According to peak area value of Catalpol,Rehmannioside D,Leonuride in drug-containing serum catalpol as an indicator to determine the best administration style and blood collection time. According to peak area value of Catalpol in drug-containing serum catalpol as an indicator to determine the best dosage. By comprehensive analysis, the dosage regimen is as following: to continuously dose for 3 days one time a day by the concentration of 0.7g/ml, 1ml/100g. To collect blood after one hour of dosage, we can examine the destination ingredients. Thus, it can lay the foundation of further research on the Serum pharmacochemisty of Rehmannia glutinosa Libosch. In the fourth chapter,we using the method of serum pharmacochemisty and analysis the compounds of Rehmannia glutinosa libosch absorbed into the blood,lays the foundation for the Rehmannia glutinosa libosch definite active constituent. The constituents absorbed into blood were determined by comparing the HPLC fingerprints of the reference substance,tested serum samples and blank serum sample. Two compounds absorbed into blood were detected, they were original constituents of Rehmannia glutinosa libosch(Catalpol,Rehmannioside D). Two constituents absorbed into blood were possible bioactive components of Rehmannia glutinosa libosch.In the fifth chapter,we investigate the parameters of serum pharmacochemistry of ethanol extract of Radix Rehamannia and Iridoid glycosides. The constituents absorbed into blood were determined by comparing the HPLC fingerprints of the reference substance,tested serum samples and blank serum sample. Three compounds absorbed into blood were detected, they were original constituents of Rehmannia glutinosa libosch(Catalpol,Rehmannioside D,Leonuride). Three constituents absorbed into blood were possible bioactive components of Rehmannia glutinosa libosch, Its serum pharmacochemistry should be subject to complete investigation so as to lay a foundation for the further study of active ingredients of Rehmannia glutinosa libosch.