Study On Veterinary Penicillin Residues And Adsorption In Soil Of Inner Mongolia Pastoral

Inner Mongolia has been one of the major livestock breeding base, with the development of animal husbandry, veterinary drugs are widely used, while the antibiotic is the most widely used veterinary drug. Antibiotics in the environment will lead to bacteria resistance in nature, and make the surrounding environment of the farm become not only a reservoir of resistance genes, but also a media of resistance gene expansion and evolution. The introduction of these exogenous compounds will bring risks to the ecological and environmental safety. Study on environmental behavior of penicillin provide scientific basis for autonomous regions pastoral antibiotics pollution prevention and control, remediation of polluted soil and environmental management, expecting to create a safer and more suitable ecological environment for sustainable development of animal husbandry in Inner Mongolia. In this paper, penicillin is used as the target pollutant to study its residues, distribution and environmental behavior in soil. The main work consists of four parts:(1) The content of penicillin was determined by HPLC. Measurement condition: 0.05mol/L KH2PO4-methanol (40:60) as mobile phase; detection wavelength with 215 nm. The preliminary results show that the degradation rate of penicillin is increasing with increasing of pH value. Place penicillin solution for a long time will generate degradation products.(2) Extraction and pre-treatment methods of penicillin in soil. Water is used as extraction agent, extract with ultrasonic. The sample was purified by SPE column and detected by HPLC. The recovery was between 75.1% and 81.5%, with RSD between 2.10% and 3.71%(n=6). The results of 10 soil samples from Inner Mongolia for residue analysis show that the detection rate of penicillin in soil samples was 50%, with detection range between 0.62 and 1.88mg/kg.(3) Study adsorption kinetics of penicillin in the soil and other influencing factors on soil adsorption. The results show the adsorption of penicillin in soil can reach equilibrium in 24h. Penicillin soil adsorption process is fit for Lagergren adsorption kinetic equationsâ…¡. The adsorption isotherms are all fit for Freundlich equation. With the decreasing of particle size, increasing of solution-to-soil ratio and increasing of organic matter, adsorption of penicillin on soil enhanced. Temperature, soil sterilization, acid and base environment were not conducive to the adsorption of penicillin.(4)The effect of different concentrations of anionic surfactant SDBS and cationic surfactant CTAB on soil adsorption of penicillin was preliminarily studied. The results show Cationic and anionic surfactant will affect the soil adsorption and desorption of penicillin.