Studies On Synthesis, Characterization, Biological Activity And Application On Purification Of Calcium-Genistein Complex

Complexing action is universal in natural druggery,and it is reported that the biological activities of metallic complex is obvious stronger than single component due to the concordance effection.The genistein is a kind of effective component from natural druggery, which has many pharmacological actions and biological activities such as antioxidation, anti-flammatory,antiviral. It have weak estrogen activeness and it can prevent and suppress each kind of tumor, the osteoporosis the woman menopause syndrome and cardiovascular disease effectively. Due to a convenient position of the 5-OH and 4-CO groups in a molecule,the genistein's structure composes a largeπ-bond conjugation system,of which the oxygen atoms has a potential activity to chelate with metal ions. It will contribute to be a new pharmacological effect or to increase their efficacy when genistein chelated with some microelements that have bioactivity and pharmacological activity themselves. Therefore, the research of genistein metal complexes, will help us to develop and utilize genistein from a new viewpoint of novel natrue compound and metal-based drug.To design some new type antioxidants of metallic complex of genistein with higher activity, using genistein and calcium hydroxide as raw material, the calcium-genistein complex was synthesized. Besides the temperature of reaction system and the reaction time the pH value of reaction solution was a key factor of the form of coordination complexes. And the optimal pH ranges were pH 8.5, the optimal reaction temperature were 65℃, the optimal reaction time were 8h. The yields of synthesizing Gen-Ca complexes were 77.42%.The calcium-genistein complex was synthesized and characterized with the methods of IR spectra, UV-spectra, thermal analysis and NMR spectrum etc. The result showed that in calcium-genistein complex Ca2+ was coordinated by 5-OH and 4-CO groups of genistein and the molecular formula is [Ca(C15H9O5)2]·2H2OIn this paper, structure-bioactivity and cooperative action for coordination complexes had been studied through analyzing the antioxidant activity of Gen-Ca complexes. The biological activities of the complex were studied by means of pyrogallol autoxidation, orthophenanthroline photometry and DPPH free radical. The result showed that at the same concentration, the scavenging effects of Gen-Ca on superoxide radical was stronger than Gen. The scavenging effects of the ligand itself and the complex increased along with the concentration increasing, and the anti-hydroxy free radical activeness elevated along with the concentration but had a maximum value, but anti-DPPH free radical ability was not so obvious.And also the bacteriostasis activities of the ligand and its complex against three different types of bacteria namely Escherichia coli, Pseudomonas aeruginosa, Listeria monocytogenes were studied. The result showed that the ligand and its complex had certain bacteriostasis effect, and the complex showed an increased activity compared to the respective ligand. Along with concentration increased, the bacteriostasis effect was obvious, and the Listeria monocytogenes was the most.Using the principle of genistein-Ca complex, we carried on the purification to 30% soybean isoflavone powder, simultaneously inspected that the optimum condition of solvent regurgitant extraction established through the single factors and quadratic rotary combination design. The optimal was that material rate was 42:1, the extraction time was 3.7h, the extraction temperature was 49℃, and the yield was 93.12%. We successfully separated the genistin and daidzin which was the main component of 30% soybean isoflavone powder. It had further important significance for preparing genistein and daidzein.It will supply the valuable referenced information for the further development and utilization of genistein and exploiting some new type anticancer medicine.