Study On Extracting, GC/MS Identification And Application In Cigarettes Flavoring Of Volatile Oil From Flos Lonicera

In this paper, four extracting methods of the essential oil in Flos Lonicera were designed and studied by the orthogonal and Box-Behnken experiment, and the optimum extraction technology of four extraction methods was defined applying the conjunction Extreme differences method with the analysis of variance and RSM. Under the optimal extraction conditions, the chemical composition and structure of the essential oil extracted in different methods were compared and analyzed using GC-MS method, and extraction of the four methods on the volatile oil components were classified and analyzed. The quality of the essential oil in Flos Lonicera extracted in different methods was also studied from the perspective of molecular. Finally, the essential oil in Flos Lonicera extracted in the optimal extraction conditions was applied to the research of cigarettes as a tobacco flavoring. The main results were as follows:1. Research on four methods of the essential oil in Flos Lonicera extracted by the orthogonal and RSM experiment.The volatile oil extracted from Flos Lonicera through steam distillation was light-yellow, semi-solid, special flavor and the 0.0563% average yield. The optimum condition was Honeysuckle cutting, the ratio of solvent to solid of 10:1, soaking 2h, distillation 10h.The volatile oil extracted from Flos Lonicera through homogenate extraction was green- yellow, solid google form, low special flavor and the 0.1756% average yield. The optimum condition was extraction reagent of n-hexane, extraction voltage 151.61v, extraction time of 45.16s, ratio of solvent to solid of 9.77:1.The volatile oil extracted from Flos Lonicera through microwave assisted extraction was yellow-green, dense google forum, low flavor and the 0.2173% average yield. The optimum condition was extraction reagent of ethyl acetate, extraction power of 632.56 W, extraction time of 19.12min, ratio of solvent to solid of 6.55:1.The volatile oil extracted from Flos Lonicera through enzymaticassisted ultrasonic methods was yellow, oily semi solids, concentrated flavor and the 0.5728% average yield. The optimum condition was enzyme dosage of 4.01% (cellulase: pectinase=0.5:1), reaction temperature 50.41℃, pH 4.51, enzyme extraction time 5.58 h. After enzyme inactivation, ultrasonic power 100.39 W, liquid ratio of 11.76:1, ultrasonic time 34.55min.Honeysuckle essential oil of different extraction methods results show that the volatile oil from the honeysuckle appearance, flavor and yield of view, enzyme assisted ultrasonic extraction method was the best extraction, the process conditions provided reference data for industrial production. Response surface method can be carried out within the continuous analysis, 3D grapH and contour lines visual performance can be obtained the relationship between factors and oil yield, interaction between factors, can accurately determine the optimum process parameters, better fitting model realistic, and better than the orthogonal design. 2. The constituents and structure of the essential oil in Flos Lonicera extracted in different methods were analyzed by GC-MS method on the condition of the optimal extraction.The four extraction methods of volatile oil of the total main components contain machine acids, esters, alcohols, aldehydes, alkyl olefins, ketones. Among them, the highest acid content, esters second, followed by esters, alcohols, aldehydes, alkyl olefins, ketones, fluoranthene and pHenanthrene were the lowest. The honeysuckle in the identification of the essential oil had a higher content of hexadecanoic acid, hexadecanoic acid ethyl ester, linoleic acid, 9,12,15- tadecatrienoic acid methyl ester, tetradecanoic acid, 9,12 - octadecadienoic acid ethyl ester, Pentac0sane, 2-flavanone and lauric acid, also contains linalool, leaf alcohol,α-Cedrol and ketone, thin class and other important aroma chemical composition. Among four kinds of extraction method the most volatile oil of components honeysuckle, the ultrasonic assisted extraction of enzyme had the most volatile oil components honeysuckle. It is for the combination of flavor, the formation of the characteristics of essential oil of honeysuckle fragrance.Under optimum conditions, the pHysical and chemical properties of honeysuckle essential oil has some differences in the different extraction methods, but the honeysuckle essential oil of the pHysical and chemical parameters changed little.Relative density0.931~0.959, less than 1, lighter than water; PH5.907~ 6.503, weak acid; acid in 29.44~36.29 mg.g-1, the refractive index 1.479~1.501.3. Research on the essential oil in Flos Lonicera extracted under the optimum conditions applied to the experiment of single and formula tobacco flavoring.The result indicated that the taste of cigarette could be improved and modified by increasing the essential oil in Flos Lonicera in right amount obviously. It had the function of lowering the roughness, improving the taste, reducing stimulation, and removing the offensive odor. The impact on the density, the strength, the flammability, and the gray of the smoke was little. It was one kind of ideal natural spice for cigarette, the rational consumption of the essential oil in Flos Lonicera in tobacco flavoring was 0.15%-0.20%.