| Objective: The first aim of the study is to optimize the extraction process and purification process of water-soluble active ingredient of Ferula sinkiangensis. The second aim is to separate the effective portion and confirm the components from the chloroform portion .Methods:①we set up the colligation scoring system which can reflect the change of the extraction rate and the contents of ferulic acid. Then the colligation score was used as the optimization index and the uniform design was used to optimize the extracting process.②The mouse model of anti-gastric ulcer were selected as indexs of separating. The polarity portions were separated by the system-solvent methods and the effective portion was founded.The silica gel column chromatogram, preparation thin-layer chromatography was used as separating method to study the components of the chloroform portion.③Stablished the fingerprint fiture with RP-HPLC and gradient washout syetem.④The extraction rate of solid was used as measured index. We use high speed centrifugation and ZTC clarifier to purify the Fenda sinkiangensis K.M. water extract respectively. To acquire the proper process condition, the gastric ulcer inhibition ratio is used to check the influence of the purification process.Compared the fingerprint fiture and check the result.Results:①The optimized extracting process is as following: adding 8 times water and extracting 2 times, every time lasting 2.5 hours.②Their structures were elucidated by chemical and nuclear magnetic resonance spectrum methods. Seven compounds were identified as famesiferol B,famesiferol C,7-OH coumarin .③Ferulic acid, F-2 were used as reference substances,Diamonsil TM C18 (250 mm×4. 6 mm ) column was used and the methanol (A) and water(B)as the mobile phase: 0~5min,90%B;5~15min,90~82%B;15~25min,82~72%B;25~40min,72~42%B;40~50min,42~32%B;50~60min,32~26%B;60~80min,26~20%B;The colum n temperature w as 40℃and the fingerprint was detected at 322 nm ; The detection time was 80 min, and the flowrate was 1. 0 mL /min, sample size was 20μl .④The optimized centrifugation purificating process is as following: concentration ratio is 1:3, centrifugal time is 35 minutes, and rotation speed is 4000r/min. The optimized ZTC1+l-Ⅱpurification process is as following: concentration ratio is 1:22,the content of pacificator is 22%, the temperature of adding B is 70℃, the temperature of adding A is 50℃, Compared the fingerprint fiture time is 1 hour. It was proved that the high speed centrifugation method is more suitable to purify the water extract according to the result of the pharmacodynamics experiment and the fingerprint fiture.Conclusion: The optimized extracting process is available, reasonable, and reproducible. Seven compounds were identified as famesiferol B,famesiferol C,7-OH coumarin. The results of pharmacodynamic test show that centrifugation purification process is suitable to the purification of extraction liquid. The method of RP-HPLC fingerprint fiture is simple and accurate with a good reproducibility and provides a reference standard for the quality control of Ferula sinkiangensis.
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