Study On Extraction,Purification,Determination Method,Biological Activities Of Ferula Sinkiangensis For Ferulic Acid

Ferulic acid is one of the effective components of ferulic acid,angelica,safflower and other traditional Chinese medicines which is widely used in food,health care products,medicine and other fields and has great market application value.However,as ferulic acid molecules contain double bonds,ferulic acid is not very stable and its solubility in water is low which greatly affects the biological activity of ferulic acid.Therefore,structural modification of ferulic acid to synthesize ferulic acid derivatives attracts the interest of many scholars.In this study,ferulic acid(ferulic acid)was extracted from Ferula sinkiangensis(feroicacid)as the research object.the crude extract of ferulic acid was further purified by macroporous resin adsorption method,and ferulic acid glucose ester was synthesized by modifying ferulic acid with glucose.the antibacterial activity and antioxidant activity of purified ferulic acid and ferulic acid glucose ester were preliminarily studied and compared.The following are the results of the study:(1)To investigate the influence of different extraction methods on the extraction rate of ferulic acid in Ferula.Ferulic acid in Ferula was extracted by ultrasonic,microwave,ultrasonic microwave enzymolysis and ultrasonic microwave alkaline hydrolysis respectively.taking the extraction rate of ferulic acid in the extract as an index,orthogonal design and response surface design methods of different factor level design methods were adopted to compare the extraction rate of ferulic acid under different methods.The results showed that the extraction rate of ultrasonic microwave alkaline hydrolysis was the highest.The optimum extraction conditions were as follows: Solid-liquid ratio was 1:8 g/mL,extraction temperature was 51.8? and extraction time was 4.49 min and the actual extraction rate of ferulic acid was 8.11 mg/ g.(2)The macroporous resin was used to separate and purify ferulic acid from Ferula and the optimal purification process was screened out.In the experiment,macroporous resin D101 was used to investigate the sample loading concentration,loading flow rate,loading PH and other factors.Finally,the optimal process conditions were determined as follows: ph = 6,flow rate 5 ml / min and sample concentration 0.4 mg/mL.The results of three groups of validation experiments showed that the purification was carried out according to the best combination of orthogonal experiments and the average adsorption rate was 96.9%.The purity of ferulic acid obtained by crystallization of eluent is 63.5 %.(3)Glucose was introduced into purified ferulic acid molecules and the structure of ferulic acid was modified.On the basis of single factor experiments,taking the conversion rate of ferulic acid as an index,the optimal process conditions for synthesizing ferulic acid glucose ester were obtained as follows: tert-butanol was used as the reaction solvent,the molar ratio of acid to sugar was 1:1,the amount of enzyme was 20 % and the reaction temperature was 58 ? for 32 h.Under these conditions,the conversion rate of ferulic acid could reach 37.0 %.(4)By comparing the antioxidant activities of purified ferulic acid,ferulic acid glucose ester and VC in vitro,the results showed that ferulic acid glucose ester and ferulic acid were effective antioxidants.At the same time,it was found that purified ferulic acid and ferulic acid glucose ester had certain inhibitory effects on Escherichia coli,Bacillus subtilis and staphylococcus aureus through preliminary antibacterial activity research,the antibacterial activity and antioxidant activity of ferulic acid glucose ester were larger than ferulic acid.