Study On The Screening Of The Strains And Role Of Conditions To Improve The Stability Of Anthocyanins From Lonicera Edulis Turcz

Lonicera edulis Turcz belongs to the Caprifoliaceae and Lonicera L. in plant taxonomy. Proved by the clinical trials, there was a certain curative effect on the cardiovascular and cerebrovascular diseases. Further more, it showed the ability of resisting some viruses and tumors, and improving the antidotal function of liver. Because of the higher edible and medicinal value, anthocyanins extracted from Lonicera edulis Turcz is widely applied in food, beverage and cosmetics. Anthocyanins from Lonicera edulis Turcz maintain a bad stability and easily influenced by the external factors such as light, temperature, pH, etc. To improve its stabilization and applied scope, the methods such as co-pigments and microtransformation were investigated in this paper.The main research contents and results in this thesis are as follows.(1)A mutant strain Candida saitoana SX-1160 which can improve the stability of anthocyanins from Lonicera edulis Turcz was obtained by means of pulse light, ultraviolet and diethyl sulfate alternately treatment. The anthocyanins showed the high preservation rate of 69.63% by bioconversion of strain, compared to 44.67% that of the anthocyanins prepared without strain, the stability had increased to 1.6 times. This study was useful for the application and development of anthocyanins from Lonicera edulis Turcz.(2) By investigating all the condition of microbial fermentation, the optimum technology for preparing Candida saitoana SX-1160 was determined, which was considered to be at 32℃, the pH of 4.0 time of 72h, and the concentration of culture medium sugar of 10Bx and for 110 r /min, ventilation of 60mL.(3) In order to detect the enhancing stability of anthocyanins, several kinds of organic acid, metals, carbohydrate or amino acids were added into anthocyanins from Lonicera edulis Turcz as the co-pigmentation. The results indicated that para-hydroxybenzoic acid (PHBA), natrium salicylicum(NaSA) and aminoacetic acid (Gly) made a hyperchromic effect and the absorption spectra of anthocyanins was bathochromic shifted, after continuous high temperature and illumination treatment remained high preservation rate. The optimum concentration of the three kinds of co-pigments were firstly selected by single-factor experiment, then the optimum conditions were determined by the method of response surface analysis with three factors and three levels. The anthocyanins prepared by adding 0.063% PHBA,0.041% NaSA and 0.039% Gly (w/w) met the highest preservation rate of 88.01%, compared to 48.97% that of the anthocyanins prepared without co-pigments, the stability had increased 1.8 times. This study was useful for the application and development of anthocyanins from Lonicera edulis Turcz.(4) Based on the optimum microbial converting conditions in pH value, relative enzyme concentration and temperature, respectively, averaging 35.5℃,119,3.20, the absorb light value come out to be 1.971, for stability testing, all the samples were put in 50℃incubator with light shine for 72 hours, then the absorb light value was determination. The anthocyanins met the high preservation rate of 89.85% by grading-up, compared to 45.32% that of the control sample, the stability had increased to 2 times.