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Studies On The Processing Technology And Properties Microencapsulation Of Anthocyanin From Berberis Kaschgarica Rupr

Posted on:2012-02-27Degree:MasterType:Thesis
Country:ChinaCandidate:J D HuangFull Text:PDF
GTID:2131330332489669Subject:Biochemistry and Molecular Biology
Abstract/Summary:PDF Full Text Request
In this paper, the study on Berberis kaschgarica Rupr as microcapsules of anthocyanins of process and anthocyanin stability and antioxidant activity have been investigated. The research on microencapsulation of Berberis kaschgarica Rupr anthocyanins has been carried out using single factor method and orthogonal rotation. The results showed that the optimum conditions of prepared microcapsules of anthocyanin of Berberis kaschgarica Rupr was that anthocyanin: wall material is 1:5, maltodextrin:β-cyclodextrin was 1:6, solid content of 30%, emulsifying agent of gum arabic 1.0%, spray drying inlet temperature 160℃and outlet temperature 80℃with good encapsulation efficiency and physical and chemical properties.The stability of anthocyanins from kaschgarica Rupr and microcapsules of anthocyanins of Berberis kaschgarica Rupr has been studied using UV-visible spectrophotometry. The results showed that anthocyanins of Berberis kaschgarica Rupr and microcapsules of anthocyanins from Berberis kaschgarica Rupr were steady in sucrose and glucose. Microcapsules of anthocyanins of Berberis kaschgarica Rupr had better state in pH value, direct sunlight treatment, indoor natural light treatment, temperature influence. Microcapsules of anthocyanins has better state than anthocyanin in K+,Na+,Al3+,Cu2+,H2O2,NaHSO3 solvent system.Microcapsules of anthocyanins of Berberis kaschgarica Rupr had better controlled release in artificial gastric juice, which completely released about 50 min in the artificial intestinal solution without sustained controlled release action. Because the structure of the anthocyanin was destroyed in the artificial intestinal solution, the result showed that the microcapsules of anthocyanins of Berberis kaschgarica Rupr had a certain effect in controlled release.Anthocyanins of Berberis kaschgarica Rupr and microcapsules of anthocyanins from Berberis kaschgarica Rupr have been investigated in antioxidant capacity Using two kinds of free radical scavenging DPPH and ABTS method. The measured anthocyanin half inhibitory concentration IC50 was 0.2644mg/mL using DPPH radical scavenging method; microcapsules of anthocyanins from Berberis kaschgarica Rupr half inhibitory concentration IC50 was 1.5027mg/mL; half anthocyanins inhibitory concentration IC50 was 1.3064mg/mL using ABTS radical scavenging method; microcapsules of anthocyanins from Berberis kaschgarica Rupr half inhibitory concentration IC50 was 4.0067mg/mL. The results showed that anthocyanin had better oxidationresistance than that of microcapsules of anthocyanins from Berberis kaschgarica Rupr, but microcapsules of anthocyanins from Berberis kaschgarica Rupr was better for storage and extended shelf life.
Keywords/Search Tags:Berberis kaschgarica Rupr, Microcapsules, Spray drying method, Quality evaluation
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