Research On Application Of Ammonia Chemiluminescence Transducer In Biosensing Technology

Chemiluminescence(CL) biosensor has already been a hot area of analytical chemistry because of its high sensitivity, good selectivity, simple measure device and so on. In this thesis, L-glutamine and L-glutamate chemiluminescence biosensor were developed based on ammonia chemiluminescence transducer; A rapid and sensitive post chemiluminescence (PCL) determination of urea with the flow injection technique was established. It was based on the PCL reaction of urea in N-bromosuccinimide-dichlorofluoresceinsystem (NBS-DCF) system. The possible PCL mechanism was proposed. These studies have some theoretical and practical signification for the exploration of new methods in the field of CL studies. The thesis was divided into three chapters and the author's main contributions as follows:1. A summary on the progress of chemiluminescence transducer in biosensing technology was presented. The first chapter mainly introduces the research contents and significance of selecting the topic.120 references were cited.2. A type of high sensitivity ammonia chemiluminescence transducer was developed based on the PCL system of NBS-DCF-ammonium. The PCL intensity responded linearly to the concentration of ammonium in the range 3.0x10-11-1.0x10-7 g/mL. The detection limit of this chemiluminescence transducer was 1x1O-11 g/mL ammonium with the relative standard deviation (R.S.D.) of 1.4% for 1.0x10-9 g/mL ammonium (n= 11). This method had been applied to the determination of ammonium in real water samples, and the results were accorded with the colorimetric method.3. A novel method based on natural animal tissue porcine kidney as recognition element for chemiluminescence sensing of L-glutamine was proposed in this section. The transducer of biosensor was based on the post chemiluminescence (PCL) system of NBS-DCF-ammonium. The signal of this biosensor responded linearly to the concentration of L-glutamine in the range 6.0x10-8-1.0x10-5 g/mL. The detection limit of this biosensor was 2x10-8 g/mL L-glutamine with the relative standard deviation (R.S.D.) of 2.1%for 5.0×10-7 g/mL of L-glutamine (n=11). The established method showed characteristics of high sensitivity, simple device and stability. This method had been applied successfully to the determination of L-glutamine in human plasma with the recovery test. 4. In this study, a new CL flow-through biosensor for L-glutamate was established by immobilizing GLDH on the eggshell membrane with glutaraldehyde as a cross-linker. The response mechanism of the biosensor was discussed. The intensity of the biosensor responded linearly to the concentration of L-glutamine in the range 8.0x10-7-8.0x10-9g/mL with a detection limit of 3×10-9 g/mL. The R.S.D. of the proposed method was 1.8%for 5.0x10-8 g/mL of L-glutamine (n= 11). This method had been applied successfully to the analysis of L-glutamate in human plasma.5. A new post chemiluminescence (PCL) determination of urea with the flow injection technique was established. The possible PCL mechanism was proposed. The PCL intensity responded linearly to the concentration of urea in the range 2.0x10-9 to 1.Ox1O-6 g/mL with a detection limit of 7x10-10 g/mL. The R.S.D. of the proposed method was 1.3% for 1.Ox1O-8 g/mL urea (n= 11). This method had been applied to the analysis of urea in milk, and the results were accorded with the colorimetric method.