| In this paper, we summarize the discovery, development, basical principle and development of resonance light scattering. What's more the cation surfactant and medicine molecular were detected using resonance light scattering. Its applications in the areas of food analysis, medicine analysis, biological science and environmental monitoring has attracted widespread attentions in recent years.Cation surfactant with both hydrophilic groups and pro-oil groups plays a great role not only in industry but also in our daily life. Because of its special bifunctional structures, cation surfactant could selectively absorb on the surface between gas-liquid, liquid-liquid or solid-liquid even in extremely dilute solution, which greatly changed the surface and interface energy of the system. However, over use and inappropriate dispose measurement of surfactant result in mass of surfactant flow in the system of water, which badly pollute and destroy water environment and organism. So the detection of surfactant content in water is a great challenge for scientists today. According to some reports, dye colorimetry can always be used to determine the content of surfactant, although its own color generally influence the detection limits. The chloroplatinic acid anion was applied as resonance light scattering (RLS) probe in this assay, and three kinds of cationic surfactants, cetyltrimethyl ammonium bromide (CTMAB), cetyl trimethyl ammonium chloride (CTMAC). cetylpyridinium bromide (CPB) were determined separately in HCl-NaAc (pH = 2.0) buffer solution, The reaction between chloroplatinic acid anion and cationic surfactants was due to electrostatic attraction, which can form a stable ion-association complex, thus the RLS intensity of system increased, compared with increase concentration of the probe and cationic surfactants respectively.The characteristic peak was located at 302nm. We also optimized the experimental conditions including system acidity, the concentration of probe, reaction time and temperature. Under the selected conditions, the enhanced RLS intensity showed a good linear relationship with the cationic surfactants concentrations in the range of 0.25×10-5～1.75×10-5 mol/L for CTMAB,0.15×10-5～2.50×10-5 mol / L for CTMAC and 0.10×10-5～1.75×10-5 mol/L for CPB, respectively, the detection limits for CTMAB, CTMAC and CPB were 4.01×10-8,4.06×10-8 and 4.72×10-8 mol/L. The correlation coefficient is 0.9987,09996 and 0.9980 respectively. The effect of some coexistent substances was also carried out. Some real samples were analysed by the RLS method. The samples were also analysed by spectrophotometry for contrast. The results obtained were satisfiedGold colloids have unique properties, such as size- and shape-dependent optical and electronic features, a high surface area to volume ratio, so now gold colloids are heavily utilized in chemistry, biology, engineering, and medicine. With increasing maturity of nanotechnology,gold nanoparticles have broad development in the field of electronics and supramolecular.Gold nanoparticles (gold NPs) have a strong surface plasmon resonance absorption peak, and the peak position exhibit a great difference as for gold NPs possess different size and shape. Gold NPs connect with biomolecule and organic molecule depend on electrostatic attraction, the aggregation have greater size than single and show a more strong scattering peak.Carbimazole is commonly used drugs of effective treatment for hyperthyroidism disease.In this paper, we ued RLS spectroscopy to study the interaction between thiol-containing molecule and gold NPs. At pH 4.0, the resonance light scattering spectrum of gold NPs has maximum peak at 580.Onm and the RLS intensity is enhanced by trace amount of carbimazole due to the interaction between carbimazole and gold NPs. The binding of gold NPs to carbimazole results in ligand-induced aggregation of gold NPs, which was characterized by RLS spectrum, ultraviolet-visible (UV-Vis) spectrum. The effect of system acidity, temperature and reaction time was studied. Additionally, we also measured the influences of various foreign substances inchluding metallic ions and biologic macromolecules. There is a linear relationship between the IRLS at 580.Onm and the content of carbimazole in range of 8×10-8mol/L-4×10-7mol/L, and could be expressed asΔIR1S=105.77453+1.08295×109C (mol/L,r=0.9983) with a limit of detection of 5×10-8mol/L(14.9ng/mL).Accoding to above all, we expect that it will become a promising technique to determine carbimazole in situ since the proposed method reported here is highly rapid, sensitive, selective and visual towards thiol groups.
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