Study On The Detection And Degradation Of Deoxynivalenol In Meat And Meat Products

Mycotoxins of animal products, which mainly due to mold contamination of feed, feeding into the body of livestock and poultry, residing in animal products will be caused of food safety problems. Deoxynivalenol (DON) which is a trichothecene of toxins, mainly by Fusarium graminearum. DON has cytotoxicity and teratogenicity, extensive contamination of food, fodder and other crops and products of plant origin, and because of contaminated feed and indirect to pollution of animal origin products. DON caused serious harm, so the method for detection and degradation is necessary.In this paper, meat and meat products as the research object, established of which by high performance liquid chromatography and gas chromatography method for the detection of DON. DON were studied in vitro degradation. The main experimental results are as follows:1. In the experiment for the determination of DON in meat products by liquid chromatography conditions:Shimadzu Shim-Pak VP-ODS C-18 reversed phase column (4.6mm x 150mm,5um), water:methanol:acetonitrile (90:5:5 volume ratio) as the flow rate of 1.0mL/min, column temperature 35℃, and the injection volume 10uL, detection wavelength 218nm. Under these conditions, DON peak shape is better, the detection limit is 0.056μg/g, the recovery rate was 7.76%～86.68%, RSD<4.8%.2. Comparison of different extraction solvents, different extraction time, different extraction way and different solid to liquid ratio on the extraction of DON effect of meat and meat products. When the acetonitrile-water (volume ratio 84:16) as solvent, extraction time of 20min, ultrasonic 70w, solid to liquid ratio of 1:4, the extraction rate of the best. the response surface results show that the experimental results with the single factor is the same.3. In the experiment for the determination of DON in meat products by gas chromatography conditions:sample side temperature was 250℃, inlet temperature of 300℃. Oven temperature program:initial temperature 150℃, maintained 2min, to 20℃/min after heating to 220℃to maintain 8min, carrier gas:N2 (purity 99.999%), flow rate 0.8mL/min, split ratio 50:l,injection volume:2uL.In this condition, DON peak shape is better, the detection limit is 0.01μg/g, the recovery rate was 82.2%～89.5%, RSD<7.76%. Experimental study of the derivatization reagent N-heptafluoro butyryl imidazole derivative of the best conditions:HFBI the optimal dosage of 50uL, the reaction temperature is 60℃, reaction time was 60min. 4. With the established method of 30 meat samples actually were analyzed by HPLC and GC, the DON content of 30 samples were below detection, indicating that the extent of contamination is low.5. Experimental study of various methods on the degradation of DON:Na2CO3 in a chemical degradation, which may be due to instability caused by DON in alkaline conditions; activated carbon has good adsorption toxin; UV degradation, microwave Degradation, and papain are basically no effect on the DON, which has to a certain extent, that the DON is indeed a stable, easy to degrade the toxins.