Proteoglycan Extracting From Fungus And Its Mention Antitoxin

In the twenty-first Century, with the frequent emergence of words including safety, hygiene, health, ecology and environment, people more and more attention to food safety. Proteoglycan has hypoglycemiceffect, hypolipidemic effect, enhance immune regulation, anti-oxidation and anti-tumor activity and so on, it has broad application prospects in the study of special medicines and functional foods. In this paper, solid-state fermentation of aspergillus, niger and monascus was studied by using certain proportion mixture of wheat bran, corn meal, soybean meal and other agricultural waste as solid-state medium. Then proteoglycan was extracted from the solid-state fermentation matrix of aspergillus and fruiting bodies of Lentinus edodes (Berk.) Sing., and optimization the best extraction conditions by using orthogonal test, proteoglycan purified grade and identification method of quality were master. The conclusions of Mention antitoxin of proteoglycan were studied as follows:1. Extraction method of fungal proteoglycan:Acid extraction of proteoglycan from solid-state fermentation matrix of aspergillus was best optimize, specific the extraction method as follow: ratio of materia and liquid was 1:30, concentration of HC1 solution was 0.75 mol/L,80℃extraction 2h, extraction rate of proteoglycan from solid-state fermentation matrix of aspergillus was 10.67% under these conditions; Base extraction of proteoglycan from solid-state fermentation matrix of niger was best optimize, specific the extraction method as follow:ratio of materia and liquid was 1:30, concentration of NaOH solution was 0.75 mol/L, 60℃extraction 3h, extraction rate of proteoglycan from solid-state fermentation matrix of niger was 9.75% under these conditions; Base extraction of proteoglycan from solid-state fermentation matrix of monascus was best optimize, specific the extraction method as follow: ratio of materia and liquid was 1:30, concentration of NaOH solution was 1.0mol/L,90℃extraction 3h, extraction rate of proteoglycan from solid-state fermentation matrix of monascus was15.69% under these conditions; Acid extraction of proteoglycan from fruiting bodies of Lentinus edodes (Berk.) Sing. was best optimize, specific the extraction method as follow:ratio of materia and liquid was 1:25, concentration of HCl solution was 0.5 mol/L, 60℃extraction 2.5h, extraction rate of proteoglycan from fruiting bodies of Lentinus edodes (Berk.) Sing. was 25.57% under these conditions.2. Identification and quantification of the quality of fungal proteoglycan. Proteoglycan was purified and protein components were identified by using UV scanning and biuret, polysaccharide components were analyzed by using phenol-sulfuric acid method, and conduct quantitative proteoglycan. The absorbance was measured with wavelength of 490nm and the standard was glucose, glucose standard curve linear regression equation as follow:Y=0.196X +0.3474, correlation coefficient R2=0.999 (R2> 0.95)3. Proposed proteoglycan components do not include glucose, fructose, maltose, sucrose, lactose and other small molecule impurities. Polysaccharide components in the composition containing both ingredients and protein, and polysaccharides content were higher than the protein's, the component in each group can be determined was proteoglycan. Proteoglycans in the buffer pH7.4 simulated intestinal environment electrophoresis test is positive, that proteoglycans in the small intestine in a positive charge.4. Effects of proteoglycan of fungal on intestinal absorptivity test:the results showed that: proteoglycan has significantly hindered to the absorption of GA in the small intestine. Proteoglycan can effectively prevent harmful substances into the blood through the small intestine, thus proteoglycan has a role on protecting the physiological function of body, it can be used after appropriate treatment or extracted.5. Proteoglycan of fungi on intestinal adhesion mechanism of action studies indicate that: proteoglycan can occupy the binding sites of mucosal surface earlier than harmful substances, connection between the intestinal mucosa cells and hazardous substances, synergies between the intestinal mucosa cells and hazardous substances become weaker, and it was fracture removed easily. This information, the proteoglycan can hinder the residues of harmful substances in the intestine, has the protective effect on the intestinal mucosa.