Research And Design Of Microinjection Controller In Transgenic Microoperation System

Gene transfer is a new and widely used technology in bioscience field. At present over 80 percent of transgenic animal models are constructed by means of microinjection. The desired volume of ejected foreign gene is determined by the holding capability of the recipient cells and the actual ejected volume of foreign gene will greatly influence the survival rate and expression result. The main research works carried out in this paper are as follows:Many literatures in the fields of transgenic technology, transgenic equipment and micro flow control technology are consulted and analyzed. According to the requirements of accuracy, repeating capability, agreeability, compatibility with environment and biology substance, a kind of electrical control microinjection system with gas Nitrogen as injection pressure medium was chosen as desired.According to the flow characteristics and the tip shape of the injection pipette measurement of ejection volume and tip size of the pipette were explored theoretically and described methodically.