Digital Microfluidics Micromanipulation Injection Of Experimental Research

With the development of biotechnology and the wider application of cell injection technology, the necessity of qualified experimental means and facilities closes behind. For example, the facility of operation and fidelity of microinjection dose, and so on. Generally, quick and accurate micro operations, velocity of injection, small transfiguration of cell and microinjection dose to the degree of pL are required in executing cell injection. There are no good equipment and means to resolve these issues yet. So it's very important to research on this respect. "Digitalization of micro fluids technology" from micro system lab of Nanjing University of Science and Technology has a breakthrough at this respect.This paper is for experimental research of cell injection by using digital micro-injection device which is based on "digitalization of micro fluids technology". The micro needle preparation devices which are used in Cell Engineering and researched by micro-system lab can create micro-injection needles. The micro-injection needles are fabricated by a large number of micro-needle preparation experiments. The performances of injecting droplets are enhanced after the surface hydrophobic and wash treatment. The micro-needles' precise physical parameters are measured by image processing technology. Then, some tests are made to detect the performance about advance and retreat of digital micro-injection device. Its injection performance are researched to get different parameters of the injection, and then the factors of the injection quantity are analyzed. On this condition, author carry out micro injection experiment on three typical and different cells. Search after drive parameter of injection, and then get the parameter range of the high ration of successful injecting. Finally, confirmed that micro-injection device can inject drug into the cell by fluorescence detecting on the cell which is injected fluorescent marker.