Study On The Effect Of Plant Growth Regulator On The Quality

Ophiopogonis radix comes from the lily family genus Radix Ophiopogon japonicus (L.f) Ker-Gawl. Dried roots, with the effect of nourishing yin moisturizing the lung, tonifying stomach and promoting fluid. As famous Chinese Staple herbs, Ophiopogonis radix has been used for a long history. With its significant clinical effect, Ophiopogonis radix is now widely used in medical drugs, health food, cosmetics and other fields. Those mainly produced in Santai County in. Mianyang City, Sichuan Province in circulation accounted for about60%in the country commodities. According to our survey, we found that Paclobutrazol, CPPU and other plant growth regulators are widely used in Ophiopogon japonicus producing area to increase the yield of Ophiopogonis radix. The study of plant growth regulators on yield and quality of Ophiopogonis radix, and on medicinal residues in soil and Ophiopogonis radix has important significance for the maintenance Ophiopogon japonicus genuineness and sustainable development.This paper starts from the plant growth regulator type, application rate and mode of administration to explore the influence of plant growth regulator on Radix plant growth, yield and quality of medicines. Through continuous sampling, dynamic residual, final residues of Paclobutrazol in herbs and soil were analyzed for Ophiopogon japonicus. This provides a theoretical basis for standardized cultivation, and help to improve the quality of medicine, to ensure the safety of clinical medicine effectiveness. The main findings are as follows:1. By measuring height, crown width, tiller number, number of tubers per plant, root weight, root length, root diameter, kernel weight, etc. we assess the impact of plant growth regulators on the growth and development of Ophiopogon japonicus. The results indicate that Paclobutrazol, CPPU, Lifengling can significantly promote increased levels of stretch and leaves and root formation and value of Ophiopogon japonicus plants, and the effect closely related to of the size of the dose; the length of the root, diameter and kernel weight significantly enhance the role; while CPPU, Lifengling traits can lead Ophiopogon japonicus root to mutation, the mutation rate is between3.33%to15%, mutation rate and growth regulators is in positive correlation (low dose of Lifengling in exception).Paclobutrazol, CPPU, Lifengling can significantly improve Ophiopogonis radix yields, increasing rate is between111.5%～387.16%, and the increasing rate is closely related to dose and mode of administration, and the stimulation effect of low dose combination of CPPU and Lifengling is significant. Software analysis shows Ophiopogon japonicus biological traits (except for root diameter, plant height) significantly influence its production. Degree of impact:single plant root weight> root number> tiller number> root length> kernel weight> crown width.2. By measuring total saponins, total polysaccharides, total flavonoids and sapogenin and diosgenin to evaluate the impact of plant growth regulator on medicinal active ingredient. The results showed that the type of plant growth regulators, the dose and mode of administration makes significant differences of the chemical ingredients; Paclobutrazol, CPPU, Lifengling alone or in combination for total flavonoids, total saponins and sapogenin content were decreased, the role of intensity and dose are positively correlated; CPPU, Lifengling plays a significantly role in reducing total polysaccharide content, the role of intensity and dose are positively correlated; Paclobutrazol can significantly increase the total polysaccharide content, potency and dose is negatively correlated.Root number, root length, root diameter with total saponins and sapogenin and diosgenin more significant correlation, and the degree of impact:root length> root diameter> root number; tiller number, root number, root length has significant correlation with total polysaccharide content, and the degree of impact:tiller number> root number> root length; tuber number more significant correlation with total flavonoids. The remaining biological traits and has no significant correlation with total flavonoids.3. By timed and contimous sampling in field trial, we analyzed Paclobutrazol residual dynamic in ophiopogon and soil, established measuring mthod of Paclobutrazol content in plant and soil, and the degradation curve equation. The result indicates that the degradation of Paclobutrazol in plants and soil is in line with a kinetic equation, the half-life in plant is3.45days, and in soil5.47days.4. In this paper, we analyzed the Paclobutrazol residues in the aboveground plant, soil and herbs. The result shows that there are Paclobutrazol residues in the aboveground plant, soil and herbs. The residual amount of Paclobutrazol is positively correlated with the fertilizing amount; while CPPU and LiFengLing alone or combinination has no significant effect on the residual amount of Paclobutrazol. The combination of CPPU and Paclobutrazol showed some antagonism in residual.5. The paper uses Ophiopogonis radix appearance, yield, chemical composition (total saponins, total polysaccharides, total flavonoids), Paclobutrazol residue as the evaluation index, using the weighted score of comprehensive evaluation to get the best application project of plant growth regulator is Paclobutrazol (a g/m2) and CPPU (a g/m2) mixed application.