| The experiments was design the single-factor test, and a total of6601-day-old healthy ArborAcre broilers were selected and randomly allotted into three treatments and two controls, thetreatments were also consisted of high, medium and low levels, total eleven groups. Theexperimental material was three kinds of different antibacterial peptides. In1~6weeks, threekinds of different antimicrobial peptides were added in diets to evaluated the effects of differentantimicrobial peptides additives on growth performance, nutrient metabolic rate and slaughterperformance in Arbor Acre broilers. The blood biochemical indexes, immune function andantioxidant capability were measured to discussed the biological activity and mechanism ofantimicrobial peptides. The optimum additive and dosage of three antimicrobial peptides (A, B,C) for farms would be selected.The experimental results were showed as follows：1. Application of the different antimicrobial peptides (A, B, C) additives in the diets improvedthe growth performance of Arbor Acre broilers. The average daily gain (ADG) was increasedand the feed/gain rate at1~3weeks,4~6weeks and1-6weeks were decreased significantly(P<0.05) with supplemented three antimicrobial peptides (A, B, C) in diets. Three antimicrobialpeptides (A, B, C) supplemented in diets also improved significantly slaughter performance andmeat quality in broilers (P<0.05).2. Compared with control treatment, the concentration of total protein (TP) and albumin wereincreased and the concentration of alanine transaminase of serum (P>0.05) was decreased, andthe concentration of blood urea nitrogen was decreased significantly (P<0.05) withsupplemented three antimicrobial peptides (A, B, C) additives in the diets.3. Compared with control treatment The amount of Bifidobacteria and Lactobacillus wereincreased significantly (P<0.05), and the amount of Escherichia coli and Salmonella weredecreased significantly (P<0.05) by using the different antimicrobial peptides (A, B, C)additives in the diets. The best of effect the on performance was antimicrobial peptides (A), theoptimum supplement dosage of antimicrobial peptides (A) was250mg/kg.4. Compared with control treatment, the immune organ indices and the concentration of IgA,IgG were increased (p<0.05) with supplemented three antimicrobial peptides (A, B, C) additives in the diets. And the optimum supplement on immune parameter was Hybridantimicrobial peptides (C) with250mg/kg.5. Compared with control treatment, the activities of serum catalase (CAT),total anti-oxidationcapacity (T-AOC) were increased and the concentration of malondialdehyde (MDA) wasdecreased in different antimicrobial peptides (A, B, C) supplemented in based diets (p<0.05),and the optimum supplement dosage of three antimicrobial peptides (A, B, C) was500mg/kg.6. In conclusion, for achieved the best comprehensive application effects, the optimumsupplement dosage of three antimicrobial peptides (A, B, C) were250mg/kg.
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