Effects Of Cecropin Antimicrobial Peptide And Curcumin On Growth Performance、Antioxidant Performance、Intestinal Microflora And Immune Function、of Broilers

This experiment was conducted to determine the effects and the interactions of cecropin antimicrobial peptide and curcumin on growth performance, antioxidant performance, intestinal microflora and immune function of broilers.Three hundred and sixty male AA broilers at the age of 1d were randomly divided into 5 treatments with 6 replications of 12 broilers each. The broilers were fed with 1 of 5 diets:a basal diet,a basal diet supplemented with 35 g/t virginiamycin premix,a basal diet supplemented with 200 g/t cecropin antimicrobial peptide, a basal diet supplemented with 200 g/t curcumin,a basal diet supplemented with and 200 g/t curcumin+ 200 g/t antimicrobial peptide. Feed and water were provided ad libitum for a period of 42 d.The results showed as follows:1 Addition of curcumin and antimicrobial peptide were significantly elevated the average daily gain and the 42d BW(P<0.05),reduce the feed/gain of broilers(P<0.05),the average daily gain and the 42d BW in curcumin +antimicrobial peptide group was significantly higher than that in the other groups(P<0.01).2 Addition of curcumin and antimicrobial peptide were significantly enhanced immune organs development and elevated their weigh index(P<0.05). The thymus index and the spleen index at the age of 21 days、the spleen index and the bursa of Fabricius at the age of 42 days in the curcumin +antimicrobial peptide group were significantly higher than the control group (P<0.01).3 Addition of curcumin and antimicrobial peptide were significantly elevated the transformation rates of T and B lymphocyte in broilers(P<0.01);The transformation rates of T lymphocyte of curcumin +antimicrobial peptide group was significantly higher than that in the other groups in 42d broilers (P<0.01).4 Addition of curcumin and antimicrobial peptide were significantly enhanced the serum total protein, albumin,immunoglobulin G and the serum immunoglobulin M content (P<0.05).The serum total protein of curcumin +antimicrobial peptide group was significantly higher than that in the other groups in 21d broilers (P<0.01).5 Addition of curcumin and antimicrobial peptide were significantly reduced the content of MDA in serum of broilers(P<0.01),and elevated the activity of SOD and GSH-px in serum; the activity of GSH-px in serum of curcumin+antimicrobial peptide group was significantly higher than that in the other groups in broilers (P<0.01).6 Addition of curcumin and antimicrobial peptide were significantly reduced the pH in duodenum, jejunum and ileum (P<0.01);The length of jejunum and ileum of curcumin +antimicrobial peptide group was significantly higher than the contral groups in broilers (P<0.05)7 Addition of curcumin and antimicrobial peptide were significantly reduced the counts of escherichia coli in caecum (P<0.05),and there were no significant differences with the antibiotic group (P>0.05); and significantly elvated the counts of lactobacillus and bifidobacteria in caecum (P<0.01).The counts of lactobacillus in caecum of curcumin +antimicrobial peptide group was significantly higher than that in the other groups in broilers (P<0.05).8 Addition of curcumin and antimicrobial peptide were significantly reduced the expression level of the IL-1β and IFN-y, enhanced the expression level of the IL-4 and IL-10 in jejunum mucosal of 21 d broilers (P<0.05); significantly enhanced the expression level of the IL-1β,IFN-γ,IL-4 and IL-10 in jejunum mucosal of 42d broilers (P<0.05); significantly enhanced the contents of slgA in jejunum.