Pretreatment Of Anaerobic Fermentation Process Of Eupatorium Adenophorum And Study On Its Antibacterial Effect

Anaerobic fermentation is an effective way of resource utilization for Eupatorium adenophorum Spreng, but bioactive ingredient contains in it will bring about chronic poisoning of anaerobic microorganisms. In order to effectively improve the anaerobic fermentation efficiency and achieve resource utilization of Eupatorium adenophorum Spreng, the initial pretreatment methods of Eupatorium adenophorum Spreng was establish, in which Aspergillus niger was used as the pretreatment agent before anaerobic fermentation, and the batch and semi-continuous fermentation methods were adopted to investigate the effect of fermentation process. Furthermore, the inhibitory effect for three pathogenic fungi from the biogas slurry was showed in this research.According to the pretreatment experiment, the degradation of lignin, cellulose and volatile oil contained in Eupatorium adenophorum Spreng were effectively promoted by the biological pretreatment of Aspergillus niger. However, pretreatment time significantly impact on pretreatment process when the other factors fixed. With the method proposed in the research, pretreated for 8 days gained the best results. Total cellulose and Klason lignin content in the fresh Eupatorium adenophorum Spreng were 51.25% and 14.03% respectively, which degradation by 10.08% and 3.48% respectively after pretreated for 8 days, besides, essential oils degradation by 37.15%. Results obtained from the batch-fermentation experiment indicted that the efficiency of anaerobic fermentation and biogas potential were improved significantly, as well as the excessive acidification phenomenon was effectively avoided.Using the pre-domesticated activated sludge from Eupatorium adenophorum Spreng as inoculums was helpful to keep the semi-continuous fermentation running well. But after pretreated by Aspergillus niger, the daily production of biogas and methane produced from Eupatorium adenophorum Spreng in the anaerobic fermentation system were significantly promoted. On the other hand, the anaerobic fermentation system was more stable than the one which use fresh Eupatorium adenophorumSpreng as raw material. The daily methane yield, biogas production rate of TS and VS were increased 74.8%, 53.6% and 58.9% respectively.Results from bacteriostasis research indicated that biogas slurry from pig dung, pretreated and non-pretreated Eupatorium adenophorum Spreng showed good inhibitory effect to these three kinds of pathogen fungi. However, the inhibition ratio affected by the concentration of biogas slurry and it would increase with the slurry concentration increased. Besides, the inhibition ratio between these three kinds of biogas slurry was obviously different for the same pathogen fungus: for Trichoderma spp. and Phytophthora parasitica var.nicotianae(Breda de Hean) Tucker, biogas slurry from fresh Eupatorium adenophorum Spreng showed the highest inhibition ratio, while biogas slurry from pretreated Eupatorium adenophorum Spreng became the most effective one for Cunninghamella.