| In tradition, fruit tree breeding needs many years and high costs. Molecular marker techonlogy has been extensively developed and applicated on crops in recent years. But it is on origion phase in fruit tree breeding. Peach (Prunus persica [L.] Batsch) is a model specie for identifying and isolating agricultural important genes in fruit trees because of its high self-hybridization affinity~ large quality trait controlled by signal gene and simple heredity principle. Two quality traits of peach are controlled by gene F and f. F verse f is complete dominance. They are important in economy because they are related to the edibility and process of peach. In order to selecte breeding parents earlier and make full use of the important ecnomic traits on fruit trees. In this study, genome DNA was extracted from fresh leaves and blast of one-year-old shoot on "Jingyu"and eiwei?and their hybridization descendants with the procedure of high concentration's salt and CTAB. DNA was detected by gel electrophoresis of 0.7% agarose and ultra-violent spectrophotometer under the wave of 260nm and 280nm. Extracted DNA could be used for PCR as it's completing electrophoresis bands and 1.8 of 0D260/0D280. The optimized RAPD reaction condition for peach was established: In a total volume of 20~il, 2Ong DNA, IU Taq DNA polymerase. 0.2mM dNTPs. 2mM Mg2~, l5ng primer were contained. Special fragment 0P107-1000 was obtained by PCR~ collected by glassmilk and cloned on pUCm-T. The T-vector was translated to DH5 a of Ecoli and screened by blue and white. Recombined cloning was obtained. Plasmid was extracted by base digested with Pst I and amplified by PCR. The results of sequencing indicated that the special fragment is 1054bp .Two nucleotide sequences obtained from "Jingyu"?and "Meiwei"?were aligned. The result indicates they are identity. One pair of primers, which are 26bp and 25bp in length, was 37 synthesized. They are used to test "Jingyu" "Meiwei"?and their descendants and polymorphic was not found.
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