| Peach (Primus persica (L.) Batsch) is a popularly cultivated fruit tree in the world. Acid and Non-acid is an important trait related to the fruit quality. Molecular markers closely linked to Acid or Non-Acid would have great contributions to the peach breeding. Take the cultivars 'Jingyu'(Dd), 'Flavor'(dd) and their BCi population (69) as the materials, by means of such molecular markers as AFLP, SCAR, RAPD, this paper studied the trait D/d controlling the trait Non-Acid/ Acid.The AFLP system suitable for peach was established. Extracted by the CTAB method, after RNA being subtracted, genomic DNA was taken as the template. Being digested by EcoRI/MseI and ligated, genomic DNA was used for pre-amplification by E+0/M+C. Selective amplification primer combination patterns EcoRI+2/Msel+3 , EcoRI+3/MseI+3 were both suitable to polymorphism analysis of peach genome. The result showed that the AFLP system was can be used for the genome of peach.According to BSA (Bulk Segregation Analysis) method, the genomic DNA of six individuals were separately mixed to set the Acid bulk and the Non-acid bulk. These two bulks were screened using 128 AFLP primer combination patterns. Three AFLP markers were found to fit the proportion of inheritance character segregation. Two of which screened by ?TA/M-CTC and E-AT/M-CTA primers were linked with Acid (d), and the other screened by ?ACT/A/-CAT primer was linked with Not-acid (D).The polymorphism fragments were cloned and sequenced. The result showed that the fragments amplified by ?TA/M-CTC, E-AT/M-CTA and ?ACT/M-CAT were 140bp, 199bp and 408bp in length. Primers were designed according to the sequence, and the 199bp fragment was converted into SCAR marker.In addition, I had planed to seek the RAPD markers related to Acid or Non-acid. Though lots of RAPD primers were screened by two extreme individuals, no marker was found closely related to the target trait in case of the low ability of polymorphismdetection of RAPD.
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