Studies On In Vitro Maturation Of Porcine Oocytes In Protein-Free Media

Four experirnents were conducted in this study to determine the effect of hormonesupplements on in vitro maturation of Porcine oocytes in two culture media (PF-TCM-l99- and PF-NCSU-23). Signilicantly more Porcine oocytes reached to the MII stage when theywere cultured in PF-TCM199 in comparison wth them cultured in PF-NCSU 23. However,supplementing TCM l99 with LH (0.5mthe, FSH (0.05mg/L and LH + FSH resulted insignificanUy more oocytes matured. Addition of LH, FSH and LH + FSH to PF-NCSU 23did not hoproved the nuclear maturation of porcine oocytes. The exposure of porcine'oocytes to hormone supplements from 0 to 2() h in PF-TCM199 resulted in a higher(P<0.05) matUration rate compared to them exposing hormone supplements during thecultllre period of 20 to 48 h and 0-48h. However, when oocytes were cultUred inPF-NCSU23, there was no significant difference (P>0.05) in their nuclear matUration ratebetWeen the Periods of hormones suPPlemented. Addition of LH or LH + FSH to TCM 199in maturation resulted in significantly more oocytes cleaved (P<0.05) after IVF, but did notaffect the proPOrtion of cleaved oocytes developing beyond the 8-cell stage to>0.05).However, addition of LH or LH + FSH to e PF-NCSU23 in maturation did not affect eitherthe percentage of oocytes cleaved or frequency of cleaved oocytes developing beyond the' 8-cell stage.These results indicate that suPPlementing TCM l99 with LH and FSH in the firstPeriod of mat'Uration (0-20 h) is beneficial to the nuclear and cytoplasmic maturation ofPOrcine oocytes.