| The purpose of this study was to analyse the genetic polymorphism of blood protein of Shandong local goats (Laiwu black goat. lining grey goat. Lubei white goat, Laoshan milk goat), Boer goat and their filial generation. Five genetic markers hemoglobin, transferrin, serum alkaline phosphatase, serum amylase and serum easterase were detected by polyacrylamide gel electrophoresis method. Genetic variantions were analysed among the different breeds from the view of biochemical genetic polymorphism and was to provide scientific basis for trait marking and linkage relationship. Statistics and calculation of production performance of purebred and filial generation were used to predict heterosis so as to provide succinct and reliable theory basis for selection of crossing parents. The results were as following:First: The body size and body weight index (Withers height, Body length. Heart girth. Cannon girth. Rump length, Rump width. Weight) and blood index (WBC. RBC, HGB, HCT.MCV.MCH,MCHC,W-SCR, W-LCR, W-SCC.W-LCC) of different breeds were measured and analysed. The results showed that there are significant difference (P<0.05 or P<0.01) on body size index and blood index among different breeds and there are significant correlation on body size index except Rump girth, but some blood index had no correlation.Second: The blood protein polymorphism was studied, the results were as following1. Polymorphism is found at the Tf, Akp and Esl loci of four local goats, all of which have two alleles and two phenotypes. Hb polymorphism with two alleles and two genotypes is found only in LN. Genetic variation of Es II is found only in LH. Amy is monomorphism. There are two genotypes in B X LH and B X LBpi and three in Boer goat and BXLBF2 at Tf loci. There are two alleles and two phenotypes at Esl loci of Boer goat and filial generation. Tf and Akp loci have no polymorphism in B X LN . There is two alleles and two phenotypes at Akp loci of Boer goat and other filial generation. Hb and Amy have no polymorphism.2. The frequency of HbA of LN is 0.9667 which is the highest. At Tf loci, thefrequencies of TfA are the highest with the frequencies of (LH,JQ,LB,LN,B, B X LH,B X LN,B X LBF,,B X LBn,the same as below) 0.9333, 0.9500,0.9453, 0.9583, 0.7344, 0.8000, 1.0000,0.8889,0.7353 respectively. At Akp loci, the frequencies of Akp?are the highest with frequencies of 0.6708, 0.7958, 0.8292, 0.9661, 0.9574, 0.9487, 1.0000, 0.9354, 0.9393 respectively, The frequency of Esl is different in different breeds. The frequencies of Esl~ are the highest with frequencies of (LH, JQ, LB, LN) 0.5323, 0.5774, 0.5728. 0.6055 in local breeds while Esf are the highest with frequencies of (B, BXLH, B X LBF1,B X LBR) 0.5918, 0.5528. 0.5000. 0.6465, 0.5149 respectively in Boer goat and their filial generation. The frequency of EsPand EsPof BXLN are equal. The frequency of Es ir of LH is 0.7418 which is the highest.3. Genetic variation of LN is the lowest but LH is the highest of purebred. The average gene heterozygosity ( H ) are 0.3547, 0.3012, 0.2892, 0.1719, 0.3238, 0.3039,0.1667.0.2585, 0.3343 and effective number of alleles (Ne) are 1.5497, 1.4310, 1.4069, 1.2076, 1.4789, 1.4366, 1.2000, 1.3486, 1.5022 respectively. The proportion of polymorphisms (Ppoly) are 0.6, 0.6, 0.6, 0.8, 0.6, 0.2, 0.6, 0.6, 0.6 respectively. Shannon information index are 0.7721, 0.2702, 0.4259, 0.4515. 0.6883, 0.6686,0.3333,0.5953, 0.6768 respectively.4. The coefficient of genetic divergence among the five purebred goat is 0.02164.This indicated that 97.386% of genetic variations of all those five goat breeds resulted from the genetic polymorphism within breeds and 2.614% is caused by the difference among those five breeds.5. Cluster analysis based on Nei genetic distance which were calculated according to the frequencies of Tf, Akp and Esl. The results showed that in four local breeds, the genetic distance between JQ and LB is closest, the genetic distance between LH and LN is smallest.6.Individual production performance of B X L... |
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