The Study On Technique System Of Tissue Culture In Brunfesia Latifolia

The stem of Brunfesia latifolia was used as explant to establish the technique systm of tissue culture.With Orthogonal design and analysis of variance,, we wanted to find out the best medium composition and the culture process in Brunfesia latifolia. The results were as follows:(1) As inoculation explant ,the middle stem of Brunfelsia latifolia in April ,May and Octomber was better than the other parts of the stem(2) Iniation culture: medium with l/2MS,IBA0.1mg/L ,NAA0.01mg/L ,BA3mg/L and KT2mg/L was the optimal.(3 ) Subculture: With MS,IBA0.5mg/L,BA3mg/L and actived carbon 0.2% as subculture medium, the increasing coefficient in 35d was 5.92.and nurslings above 2cm were 47.16% of the differentiated total buds. Actived carbon could prompt the growth of buds. Subculture generation had an influence on propagation and differentiation of buds. In general, the sixth generation was the best.(4) Rooting culture: For rooting culture, medium with MS,IBA0.5mg/L,BA3mg/L and sugar 30 g was the best with rooting rate 88.16% and average length of root 4.96cm, while rooting substrate with 1/2 perlite ,1/2 vermiculite and ASD5 mg/L was the best, rooting rate35.76% and average length of root6.49cm.(5) Transplantion: In transplanting, the survival rate of Brunfelsia latifolia roots was higher than that of Brunfelsia latifolia with no roots . Through strong illumination and in closed bottle, the survival rate could be improved to 83.24% .Substrate with perlite and vermiculite by 1 to 1 was the optimal.