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Cytological Identification Of A Wheat-Aegilops.ventricoca Material And Localization Alien Chromatin Specifying Resistance To Wheat Powdery Mildew Resistance Genes In Wheat (Triricum.aestivum)

Posted on:2004-02-13Degree:MasterType:Thesis
Country:ChinaCandidate:H X WenFull Text:PDF
GTID:2133360092485658Subject:Crop Genetics and Breeding
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Powdery mildew, caused by Erysiphe graminis f.sp.tritici,is one of the most serious diseases of wheat (Triticum aestivwm)throughout the world, and it has become a major threat to wheat production. In view of this many professors try to find new resistance genes in Triticeae. Genetic material from Ae.ventricoca has transferred to hexaploid wheat through an intermediate self sterile hybrid between T.durum(AABB) and Ae.ventricosa(DDMvMv),which has backcrossed using pollen from common wheat (AABBDD),and the progeny were repeatedly selfed to obtain stable wheat line with 42 chromosomes, designed 26-2-15 line. The results are as follows:Two wheat/ Ae.ven/ Ae.ventricosa addition lines was characterized on the basis of morphological and cytological characters. Utilization of differential identifying techniques, morphological study,and cytological study, permits the recognization of chromosomes and/or genomes and, consequently, identifies the chromosome Mv involved in wheat (T.aestivum).The chromosome Mv in wheat(T. aetivum)have been studied by analyzing M I configurations in hybrids F5 and F6. The configurations of 26-2-15 is 18.37 IIRing+2.58 II Rod +0.08 I , and the utilization of conventional staining techniques combined to the cytological analysis makes it easy to identify the chromosomes or genomes, which are present in hybrids. The chromosome in the line 26-2-15 have been identified a 5MVL/5DS translocation chromosome by C-banding and the additional line 26-2-13 has a pair of Mv chromosome belong to Ae.ventricosa.It is indicated that the infertilization was happened in the course of DNA copying, however, not in the spore maturing.lt is estimated that the alien chromotin /or gene cause the losing chromotin in various levels.
Keywords/Search Tags:Triticum aestivum, Ae.ventricosa, Powdery mildew, resistance genes, chromosome M~v
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