Immunologic Changes And Mechanism Of Peripheral Blood And Immune Organs In Probiotical Chicks And Probiotical Chicks Immunized With ND Vaccine

In this experiment, staphytococcal protein A(SPA) rosette test, indirect enzymelinket immunosorbent assay (ELISA) and immunohistochemistry, histochemistry staining were used to research the dynamic changes of the cellular and humoral immunity in peripheral blood and immune organs of probiotical chicks and the probiotical chicks immunized with ND vaccine. In order to discovery the cellular and humoral immune enhance effect in probiotical chicks and the effect on irmiune response and mechanism of peripheral blood and immune organs in probiotical chicks to ND vaccine.The results were as follows: the number of T, B cells or the content of IgG, IgM, IgA in peripheral blood and the number of T cells or IgS antibody-producing cells in thymus, bursa of Fabricius and spleen increased in different extent as compared with those of control chicks,and the content of IgM, IgA in peripheral blood, the number of IgM, IgA antibody-producing cells in bursa of Fabricius and spleen reach the highest on 7th day after feeding with probiotics (AF) , the content of IgG in peripheral blood, the number of IgG antibody-producing cells in bursa of Fabricius and spleen reach the highest on 10th day AF. The results showed that the level of cellular and humoral immunity of the probiotical chicks could be enhanced. The number of T, B cells or the content of IgG, IgM, IgA in peripheral blood and the number of T cells , IgS antibody-producing cells in thymus, bursa of Fabricius and spleen increased significantly (p<0.05) as compared with those of ND vaccine chicks within 7 days. The results indicated that the level of cellular and humoral immune response of probiotical chicks after immunizing with ND vaccine could be enhanced . Moreover, the data of probiotics I was higher than that ofprobiotics II.It showed that the probiotics I was better than probiotics II in the respect of immune enhance effect. Probably because probiotics I contain more yeast than probiotics II led to this results.After challenged with NDV, the immune protection rate of P O, PII, ND and C group were separatedly: 100%, 100%, 80%, 0.The results showed that the probiotics could synergy ND vaccine enhance the general immune responses of body to antigen, and enhanced the immune effect of the ND vaccine. Resist effectively the attack of NDV and enhanced the percentage of protection of ND vaccine.