Effects And Mechanism Of Immunologic Changes On Peripheral Blood And Immune Organs Of Chicks Treated With High Voltage Electrostatic Field

The experiment has applied staphytococcal protein A (SPA) rosette test,indirect enzyme- linked immunosorbent assay(IELISA),avidin biotin-peroxidase complex technique(ABC)and histochemistry staining ways et al. Researching completely into the dynamic changes of the cellular and humoral immunity in peripheral blood and immune organs of chicks after high voltage electrostatic field (HVEF) having irradiated. Intend to discover the effects and mechnisim of immunologic changes on the cellular and humoral immunity in peripheral blood and immune organs of chicks treated with HVEF.The study was as follows: after HVEF irradiated chicks, the numbers of T,B cell and the contents of IgG,IgM,IgA in peripheral blood of chicks by high voltage positive electrostatic field irradiating increased more significantly than control chicks during 1 to 14 day; the numbers of T,B cell and the contents of IgG,IgM,IgA in peripheral blood of chicks after high voltage negative electrostatic field irradiating were more lower than those of control chicks during 4 to 14 day. The results showed that high voltage positive electrostatic field can enhance the level of the cellular and humoral immunity of peripheral blood during some periods , while high voltage negative electrostatic field has inhibition or negative modulation on the immune system of chicks. After HVEF irradiated chicks,the numbers of TANAE+ cells ,CD4+ and CD8+T cell ,its ratios in thymus and spleen and the number of IgG,IgM,IgA antibody-producing cells in bursa Fabricius and spleen of chicks by high voltage positive electrostatic field irradiating increased more than those of no irradiating chicks during 4 to 21 day, while above-mentioned items of chicks by high voltage negative electrostatic field irradiating were fallen more than those of control chicks. From the data can illustrate high voltage positive electrostatic field can greatly strengthen immune function of chicks, on the contrary high voltage negative electrostatic field restrain or negatively regulate it. Whether or not group treated with high voltage positive electrostatic or high voltage negative electrostatic field is irradiated, HVEF brought the increase evidently of body weight of chicks, while the body weight of chicks by high voltage negative electrostatic field irradiating was greater weight than those of chicks by high voltage positive electrostatic field irradiating. It showed that the changes of before-mentioned items have no relation with the increase of body weight of chicks.The above-mentioned results of the study for the first time explains that high voltage positive electrostatic field can advance the level of cellular and humoral immunity in peripheral blood and immune oranges of chicks, while high voltage negative electrostatic field hold back its levels. Meanwhile, it illustrated that HVEF gives rise to the inner relations and regularity on immune changes of chick, in order to further discover the detail mechanism of immune changes of chick treated with HVEF and then provide theoretic basis for relative reaseach. The experiment also discovered that high voltage positive electrostatic field and high voltage negative electrostatic field can bring the increase of body weight of chicks, while high voltage negative electrostatic field has even more evident effect than high voltage positive electrostatic field'.