Study On The Screening Synergistic Bacteria Of Tobacco And The Effects Of Bacteria On Internal Quality Of Tobacco Leaves

This research content is to improve tobacco quality by bacterial strain. The bacteria were used during the growing,curing and fermenting. The synergistic bacteria would screened through the assessment with sense organ by smoking and analysis of chemical ingredient,such as soluble sugar,total nitrogen,protein,caritenoid and nicotine. Study on the mechanism of the bacterial fermentation,biological,physiological and biochemical characters,discussing fermentation parameters. The main achievements or conclusions and results are acquired as follow:1. More 240 strains were isolated from specimens of leafage and soil,which were collected from Yunnan and Shandong province. The analysis of the chemical ingredient of flue-cured tobacco leaves and the panel of test indicated that 3 strains,B07,B21 and B45 can improve tobacco quality. Aroma quality increased 3.54%,aroma quantity increased 3.13%,2.50% and 3.13%,respectively. Offensive and irritancy were reduced.2. The analysis of the chemical ingredient of tobacco leaves with strains demonstrated that 4 strains of 10 strains made in growing period,B03,B09,B07 and B05 that improve aspect quality of flue-cured tobacco leaves,and 4 strains of 28 stains were made during curing period,all could make chemical substance changed largely,and more harmonies than that of the control.3. In order to exert the role of bacterial strains in improving tobacco qulity greatly,and offer proper technical parameter,biological,physiological and biochemical characters of the strains B07,B21 and B45 were studied. Theresult indicated that strains B07 and B45 belong to bacillus,peripheral flagella,Gram-positive,no capsule,salt toleration. Gelatin liquefaction test,amyloclastic test and hydrogen peroxide test were all positive reaction. As for the strain B07,the range of optimal temperature is 25～40℃,the range of optimal pH-value is 8.0,the optimum aton is glucose and saccharose,and the optimum nitrogen source is peptone. It can not utilize sorbose. As for the strain B45,the range of optimal temperature is 25～36℃,optimum pH-value is 8.0,the optimum aton is glucose and saccharose,and the optimum nitrogen source is beef extract. It can not utilize sorbose and rhamnose. As for B21,thallus is rod,polar flagellum,Gram-negative,no capsule. Amyloclastic and hydrogen peroxide test were positive reaction. Gelatin liquefaction test,indole test and methyl red test were all negative reaction. The range of optimal temperature is 25～30℃,the range of optimal pH-value is 6.0～8.0,the optimum aton is glucose and saccharose,the optimum nitrogen source is peptone. It can not utilize sorbose.4. Study of the changes of five enzyme activities(including α-amylase,cellulase,proteinase,polyphenol oxidase and peroxidase) in flue-cured tobacco leaves during bacterial fermentation. The result showed that the enzyme activities of bacterial fementation were better than that of the control,and the assessment value have closely relationship with enzyme activities. The changes of cellulase activity were not consistent during bacterial fermentation. The changes of peroxidase activity was continuously decreased. For α-amylase,proteinase and polyphenol oxidase which were higher than that of the control,the enzyme activities ascended firstly,but respectively descended on the 3th or 9th day,...