Study On Organogenesis, Somatic Embryogenesis And Plant Regeneration In Vitro Of Plantanus Orientalis L. Leaves.

Platanus orientalis L. is one of main gardens greening tree species in our country. It has the stronger ability of cleaning air and stronger ornamental characteristic. Its wood may be used as architecture and furniture and so. It is a good quality tree species having ecological, economical and society benefit. But with tree age older, its fruit hair' contamination has become a big public nuisance. In order to study mechanics of Platanus orientalis L. sterility from theory by plant tissue culture and build a receptor system of good gene inversion for sterility trans-gene Platanus occidentalis L. culture, the paper used Platanus orientalis L. leaves as explants and studied the effects of different basic culture media, different concentration of plant hormone ,different temperature treatment, leaf position and culture time on inducing leaf morphogenesis and plant regeneration by orthogonal layout and signal factor experiment. The results showed: the best basic culture media was WPM.The best culture media of no-embryo callus induction was WPM+0.16IBA+8.06-BA.The best culture media of 20d suitable culture, embryo callus tissue induction was WPM+0.1IBA+5.06-BA.The best culture media of 25d suitable culture, no-embryo callus tissue induction was WPM+0.1IBA+5.06-BA. The best culture media of 50d suitable culture, embryo callus tissue somatic embryogenesis was WPM+0.56-BA. The best culture media of 45d suitable culture, adventitious bud induction was WPM+0.1 IBA+3.06-BA. The best culture hiedia of 50d suitable culture, the best culture media of root induction and embryoid development was 1/2MS0.1~ 0.51BA and WPM separately. 3d after inoculated leaves tissue or callus were pre-treated in 10癈 low temperature, the inducting ratio was improved differently under 25 癈 temperature conditions. The highest ratio of leaf, plant regeneration' adventitious bud induction is the fifth leaf numbering from basis to top after the seventh true leaf of germ-free seedlings unfolded. During culture, hormone IBA benefited callus induction, CTK-6-BA benefited adventitious bud induction, the effects of ZT on callus and adventitious induction wasn't significant.At first, IAA and CTK of higher concentration were needed in leaf callus induction. Their concentrations were needed to reduce in inducting adventitious. But IAA was't needed in somatic embryo44development, only needed to add CTK. Rice-yellow, tight callus was organogenesis main callus. Brown, purble-red, loose callus was the main callus of somatic embryogenesis.These results supplied basis for the culture of Plalanus occidenlalis L. new varietyby different means.