Studies On Genetic Analysis And Cross Breeding Of The Transgenic Chrysanthemum (Dendranthema X Grandiflorum Kitam)

Chrysanthemum is one of the most famous ornamental plants and has a long history of culture in China.Ground-cover chrysanthemum plays an important role in landscape. We had introduced chalcone synthase(CHS) gene into "Mei Ai Huang'.an excellent cultivar of chrysanthemum.In the world,using the transgenic technology into breeding study had been reported,but studies on cross breeding and heredity analysis of the offspring is few. In this study.through having observed growing conditions of the transgenic line 05 , 08, 33, 42 in the greenhouse,and having made the transgenic chrysanthemum as well as control separately cross with different colors chrysanthemum varieties,the subject of heredity and breeding of the introduced gene chrysanthemum were discussed.The result shows that the transgenic chrysanthemum can grow normally in the greenhouse and is not resisted on introduced the foreign gene,and can keep intrinsic excellent characters.When blossoming,different lines exist definite difference.Flower doubleness of one or two plants of line 05 changed,but most characters including (lower color were not changed.In the course of cross breeding.a multitude of chrysanthemum seeds were obtained by improving crossing technology.Seeds of the transgenic chrysanthemum F1 hybrid had high rate of germinating.not because of the introduced gene affected the vegatative growth of chrysanthemum.The transgenic chrysanthemum Fl grew well in the green house.Combining cross breeding with forcing culture in the greenhouse,the breeding of two generations will be finished in one year.Further PCR testing on the offsprings of line 08 'Mei Gui Hong' indicated the foreign gene could entailed to the offsprings by the sexual method.That is to say,two plants having yellow flowers exist the introduced gene.We can infer through analysing the inheritance of some characters of chrysanthemum that the expression of flower color of hybrids somewhat trended to that of female parents,plant height was of definite heredity advantage,the expression of flower diameter and flower doubleness had a declined Irendency.The large variety in Fl hybrid occured.The phenomena that overparent individuals of some characters in (lower color.plant height,flower diameter and flower doubleness emerging are beneficial to the choice of chrysanthemum breeding. Via estimating and choosing the offspring of chrysanthemum,some relatively good individuals had been chosen out.But the observation of the offsprings still need to be proceeded with.