Development Of PCR For Goose Parvovirus Detection And Studies Of The Distribution Of Goose Parvovirus In Artifical Infected Gosling

Primers were designed based on the complete nucleotide sequence of goose parvovirus B strain that zadori subjected to Genebank in 1995,the PCR method for goose parvovirus detedtion was developed.Using this method we can amplify a differential fragment about 441bp from GPV sample ,but we cann't get the same fragment from duck plague virus.Ecoli O1 and O8,MDPV, NGVEV samples.By this method a minimal sample of 0.47pg goose parvovirus nucleotide can be detected.Using the PCR method developed above,we studied the distribution regularity of virulent goose parvovirs in artifical infected gosling body.The result indicate that there exist difference among the three group(subcutaneously,orally,intranasally).The result of orally infected group: 4 hours after infected ,the GPV nucleotide cann't be detedted from all samples collected; 8 hours afete infected, the GPV nucleotide can be detected from heart and liver;24 hours after infected, the GPV nucleotide can be detedted from thymus,pancreas,duodenum,small intestine,bursa fabricius ,heart,liver,spleen,kidney and blood;48 hours after infected, the GPV nucleotide can be firstly detected from rectum,bone marrow and lung;72 hours after infected , the GPV nucleotide can be detected from cerebellum ,mudulla oblongata,appendix,skeletal muscle,feces.312 hours after infected the GPV nucleotide still can be detected from duodenum,jejunum,rectum,heart,liver,kidney,skeletal muscle,feces.The result of subcutaneously infected group:4 hours after infected, the GPV nucleotide cann't be detedted from all sample collected;8 hours after infected, the GPV nucleotide can be detected from heart and liver;24 hours after infected, the GPV nucleotide can be detected from mudulla oblongata,thymus,pancreas,duodenum,small intestine,appendix,bursa fabricius, heart, liver spleen,lung,kidney,blood,cerebellum,rectum,skeletal muscle,feces;48 hours after infected ,the GPV nucleotide can be firstly detected from bone marrow;312 hours after infected,the GPV nucleotide can still be detected from duodenum, small intestine,appendix,heart,liver,kidney,feces.The result of drop nose:4 hours after infected,the GPV nucleotide cann't be detected from all sample collected,8 hours after infected,the GPV nucleotide can be detected from heart and liver.24 hours after infected,the GPV nucleotide can be detected from thymus,duodenum,rectum,jejunum,lung,cardiac muscle,48 hours after infected GPV nucleotide can be detected from cerebellum, medulla oblongata, Pancreas, cecum, bursa of Fabricius, spleen, Skeletal muscles, bone marrow, feces,blood.312 hours after infected GPV nucleotide can be detected from thymus, duodenum, jejunum, cecum, rectum, cardiac muscle, liver,Kidney,feces.during the period of this studies, we haven't detected the GPV nucleotide from cerebrum,tongue,glandular stomache and gullet.83 days after infection,the virus DNA can't be detected from any tissues of goslings.liver,carciac muscle, jejunum are the most suitable tissues for virus isolation.72 hours or so after infection GPV distribute most widely in gosling.