Effect Of Ear Acupuncture On Activity Of The Nucleus Paragigantocellularis Lateralis Of Rabbit And The Possible Mechanism

Objective To investigate the mechanism of ear acupuncture by studying the adjustment of ear acupuncture on activity of the nucleus paragigantocellularis lateralis of rabbit. Methods 60 health rabbits were divided randomly into: 1. pneumocardial area group(PC group): (1) normal saline group (N group); (2) bicuculline group (B group); 2.non-pneumocardial area group (nPC group): (1) normal saline group (N' group); (2) bicuculline group (B' group). Mean arterial pressure (MAP), pulse pressure, heart rate(HR), respiratory pressure and frequency of respiration at different moment were recorded for analysis. Results _I PC group: 1.N group: When only NS was microinjected into PGL, blood pressure and respiration did not change significantly; When NS was applied into PGL with ear acupuncture in PC simultaneously, compare with the former, blood pressure and respiration did not change significantly. 2. B group: When only bicuculline was microinjected into PGL, MAP was significantly higher; Pulse pressure, HR, respiratory pressure and frequency of respiration did not change significantly. When BIC was applied into PGL with ear acupuncture in PC simultaneously, compare with the former, MAP was not so much high; The time of MAP came back to normal level, pulse pressure, HR, respiratory pressure and frequency of respiration did not change significantly. II nPC group: l.N' group: When only NS was microinjected into PGL, blood pressure and respiration did not change significantly; When NS was applied into PGL with ear acupuncture in PC simultaneously, compare with the former, blood pressure and respiration did not change significantly. 2.B' group: When only bicuculline wasmicroinjected into PGL, MAP was significantly higher; Pulse pressure, HR, respiratory pressure and frequency of respiration did not change significantly. When BIC was applied into PGL with ear acupuncture in nPC simultaneously, compare with the former, MAP was not so much high; The time of MAP came back to normal level, pulse pressure, HR, respiratory pressure and frequency of respiration did not change significantly. Conclusion 1. Normal situation of body won't be disturbed by electronic acupuncturing either PC or nPC, but abnormal activities can be adjusted by it. 2. Possibly, mechanism of electronic ear acupuncture act on GABA system of PGL. 3. It seemed that the adjustment of ear acupuncture is controlled by some factors that are unknown now; 4. PC and nPC are different areas on ear. But the difference of adjustment between them is relative.5. In addition, if PGL is involved in adjustment to respiration, GABA maybe is not a role of it.