Location Of The Fertility Restorer Gene For T-Type CMS Wheat By Microsatellite And Rapd Marker

CMS(Cytoplasmic male sterility )is comprehensive in the plant kingdom and it is the base of the crossbreed predominace using in the crop, nearly ,the genetic study about the Rf (fertility restorer) gene is hotspot. Location of the fertility restorer gene is the precondition of the assistant breeding. In this experiment,through the genetic analysis of an F2 population, derived from CMS line 75-3369A ( T-type CMS wheat) and the restorer line 7269-10, the result indicated that the restorer line is conditioned by two dominant genes.An F2 population was used to map the fertility restorer (Rf) gene by microsatellite and BSA (bulked segregant analysis). Restorer and sterile DNA pools were established using the extreme fertile and sterile plants of F: population, respectively. Among the 230 pairs of microsatellite primers, two markers were found polymorphic between the two pools. Linkage analysis showed that microsatellite marker Xgwml36 and Xgwm550 were linked with the two fertility restorer genes, respectively, with Joinmap 1. 4 (software), One of the Rf gene was located on 1AS and the genetic distance between the SSR marker Xgwml36 and this Rf gene was 6.7 cM, the other Rf gene was located on IBS and with a genie distance of 5. IcM to marker XgwmSSO .Using RAPD(random amplified polymorphic) marker and BSA loated the Rf gene . Among the 230 RAPD primes, two markers were found polymorphic between the two pools, which were OpronI04 and Opron009. The polymorphic was steady. By mapping the gene with Joinmap 1. 4 (software), the genetic distance between the the primer OpronI04 and the Rf gene was 3.9 cM; the genetic distance between the the primer Opron009 and the Rf gene was 27.8cM.