Studies On Genetic Polymorphisms Of Microsatellite DNA Loci And The Individual Discrimination And Paternity Testing Of Police Dogs

The purpose of the study about the genetic polymorphisms of 10 microsatellite DNA loci of dog is to know the relation among different dog breeds and to obtain the genetic data of 10 microsatellite DNA loci. We finally want to establish the molecular biology theory base for the breed, individual discrimination and the paternity identification of dogs via establishing the gene database of policedogs in Hubei area.Five dog breeds originating from 3 countries, Germany, United Kingdom, China were chosen for this study of genetic relationships. Genetic relationships were evaluated on the basis of 10 microsatellites loci typed in a total of 153 DNA samples collected. Allelic frequencies and average heterozygosities revealed the greatest variation in Labrador retriever and the lowest in German Shepherd dog. The phylogenetic tree displayed a remarkable degree of breed clustering and reflected an extensive underlying kinship structure, particularly for the breeds from Germany. Similarly, genetic differentiation between breeds, as measured by average FST. was highly significant and showed that 42 percent of genetic diversity in the total breeds could be attributed to differences between the breeds. Significant deviation from Hardy-Weinberg equilibrium (HWE) were shown in a number of specific locus-breed combinations. Overall relationships between breeds reflected their geographical origins and morphological data. The principal component analysis testified the results.The study of the polymorphisms of the 10 microsatellite DNA loci of the 118 unrelated police dogs which have no relationship, including 85 German Shepherd dogs, which are all from Hubei, shows that the three microsatellite DNA loci PEZ6, PEZ8, FHC2054, which all have over 8 allelic and their DP approach or surpass 0.9, heterozygosity approach or surpass 0.7, are fit for the dog's paternity identification and individual discrimination efficiently. The rest 7 loci don't satisfy the ideal demand of the paternity identification and individual discrimination.Basing on the demand of STR-PCR on template DNA, when we analyze the AFLP of the microsatellite DNA loci with large amount of samples, as the study shows, we can adopt the method that collect the oral epilhelia or blood stain and use Chelex-100 resin to extractDNA easily and quickly. Simultaneously, the use of fluorescence labelled multiplex PCR and automatic capillary electrophoresis can make the data more intuitionistic, it has the advantage of easy operation and high discrimination, and also can help to enhance information content of single detection.