| The catastrophic decline in global biodiversity has led that the last refuge for a number of organisms may be off site conservation. One of the important tools in the genetic management of captive populations is pedigree analysis, with detailed pedigrees it is possible to obtain genetic information, such as the level of inbreeding and the distribution of founder genes within the current members of the population. The pedigrees were confusion in some captive populations for breeding reason.The Amur tiger (Panthera tigris altaica) captive population of Hengdaohezi Felidae Animal Breeding Center and Ha'erbin Tiger Park are come from the same founders, that population is the most captive population in the world and is the main population for the Amur tiger conservation plan. In this study, we identified the paternity using microsatellite locus and evaluated the genetic diversity in the captive poulation.Eighteen polymorphic microsatellite DNA primers identified in domestic cat (Felis catus) were amplified in Amur tiger genomes, and found that four of the loci did not amplify product, eight were monomorphic and six (FcaOOS, Fca075, Fca094, Fcal52, Fcal61, Fca294) were polymorphic. At the same time, we designed eight microsatellite primers by the sequences of Sumatrae tiger (Panthera tigris sumatrae), the amplified result in all Amur tiger genomes showed that four primers were monomorphic and four (Pti002, Pti003, Pti007, PtiOlO) were polymorphic. So the percent of polymorphic in twenty-six microsatellite markers was 38.5%.We tested paternity and identified their biological father of 46 tigers(15 litters) who had two or more probably fathers in captive population using these ten polymorphic microsatellite loci. We calculated the excluding probability of paternity(PPE) of each locus and cumulative chance of exclusion(CCE), the value of CCE was 99.1%. We found some male tigers had higher propagate competence and some male tigers were lower.In 113 samples, we found 41 alleles and the most allele number of Fcal52 locus had 6. We calculated the gene heterozygosity and the polymorphism information content of each locus. The values of gene heterozygosity is 0.385?.707, The values of polymorphism information content is 0.353~0.658, which showed this captive population had some genetic diversity and these loci were high polymorphism.In the study, we got the hair DNA genomes used the non-invasive DNA technology. Compared the amplfied result of hair DNA with the blood DNA, we found they all had clear lines. These results imply the microsatellite DNA markers and non-invasive DNA technology can help establish the detailed pedigree and breed plan for captive population of Amur tiger. And we could use this means to other endangered species for their conservation.-2-We analysed the current situation of the captive Amur tiger population and found that we have good breeding technology. But the average coefficient of inbreeding in Tiger Park (0.1056) was much higher than Hengdaohezi(0.0292).At last, We thought that followed equality principle, exchange principle, fetch in wild tigers, prolong gap time and perfect pedigree would help avoid the inbreeding and increase the genetic diversity.
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