Research And Application Of Embryo Transfer In Cattle And Goats

The commercial application of embryo transfer (ET) in cattle and goats was restricted by the ordinary estrous synchronization, unstable superovulation and unsystematic related index. This research was supported by the Bovine Embryo Engineering Institute of NWSUAF and 7 Farms. The result was as follows:1. In the bovine superovulation procedure of Holstein by using PG, P4 and FSH:(1) The number of corpus luteum (CL) was not significant different by different time removing the CIDR-B at the 7th and 8th FSH administrition(5.5±0.5 vs 6.0±1.3, P> 0.05). The number of total embryos (4.0 ±0. 0 vs 7.5 ±3.3) and transferable embryos (3.0±0.0 vs 2.8±0.9) were not significatly difference (P>0.05).(2) The Flushing total embryos(4.0±0.0vs 7.3 ±1.9) by using different Folltropin-V dose 400 and 440 mg respectively were not significantly different (P>0.05).2. In the superovulation procedure of Boer goats by using PG, P4 and FSH, (1)In different procedures (Procedure 1 removes CIDR or PRID later 6h than procedure 2), the number of CL was significantly difference (33.3±2.2 vs 23.5±1.6, P<0.05) , the number of total embryos (22.4 ±2.3 vs 20.4 ±2.0) and transferable embryos (20.9 ± 2.0 vs 18.8 ±2.1) was not significantly (P>0.05). ㏕he results of superovulation hadn't distingushed difference using the different kinds of FSH (produced in Australia and Institute of Zoology, Chinese Academy of Sciences) and different dose(P>0.05). (3) The superovulatory results hadn't distinguished difference (total embryos was 22.4 ±2.3, 17.8±2.2 vs 19.3±5.5, transferable embryos was 20.9 ± 2.1, 16.0 ± 2.0 vs 14.6±5.3, P >0.05) from August to December in Shaanxi.3. Boer goats were used as the donors and Guanzhong dairy goats as the recipients in the study. The estrous synchronization rates among the recipient's earlier 12h, 6h and oh were not significantly different (73.8%, 80.7% vs 73.1%, P>0.05).4. In the studies of relationship between the volume of CL and pregnancy, the pregnancy rates between recipients with different size of CL were not significantly different (69.6%, 56.0% vs 71.4%, P>0.05).5. The pregnancy rate of the recipients transfered 1, 2, 3 embryos was not significantly different (78.8%, 61.9% vs 61.8%, P>0.05). The recipient's ratio of producing twin kids which transfered 2 embryos was 32.4% (55/170).6. The ovarian responses between the "white" and normal CL goats were not significantly different (the numbers of CL are 16.2±1.1 vs 20.1 ±1.1 respectively, P> 0.05). Total embryos (4.3 ±2.4 vs 17.1 ±1.5) and transferable embryos (4.3 ±2.4 vs 15.0 ±1.3) were significantly different (P<0.01). The kids of every doner were significantly different (1.7±0.9 vs 5.2 ± 0.6, P<0.05). The pregnancy rates of recipients were not affected (66.7% vs 57.5%, P>0.05).