| Maize wallay ear virus (MWEV), a kind of important maize disease firstly burst out in Sichuan province at the end of 20th century, was caused by(Cicadulina bipunctella (mats) . It damages maize in field and leads to great economic losses when the population occurs in abundance. The dominance population is ascertained by the field investigations and indoor trials, the relationships between C bipunctella and MWEV, the bio-ecological characteristics of C. bipunctella, were studied systematically. The virus was identified as MWEV, The DAS-ELISA method for MWEV detection was developed using Antiserum prepared by the purified virus and antibody, The research contents and results were listed as follows: l.The relation between MWEV and Cicadula. bipunctella (mats)The insects that collected from the MWEV disease field were transmitted to the corn. The result shows the C. bipunctella (mats) can induce the maize to the symptom of dwarf, crimpled leaf just like the symptom of MWEV. And the C. bipunctella (mats) population is the dominance leafhopper population in the field (0.0629-3.0114 per corn). And the incidence of disease was 98% by this insect. The other insects can not induce the maize to this symptom. All of this result shows that the C. bipunctella (mats) was one of the vector of MWEV.2.The propagate of MWEVThe MWEV can't be transmitted by graft, cuscuta australis R.Br, injecting while can transmitted by C. bipunctella (mats). The construction and propagation of MWEV depend on the C. bipunctella (mats). Zea mays L was conformed to the adaptive host for the leafhopper for the development, survivorship, longevity, propagation of C. bipunctella (mats) on the six host: Zea mays L. Triticum aestivum L. Sorghum vulgare Pers. Oryza saliva L. Setaria italica (L.) Beauv. Hordeum vulgare L and the maize were easy to plant, growth rapidly, the symptom obvious. The results shows the C. bipunctella dead in the 3-5 days After C. bipunctella with MWEV is transferred to N.Tabacum samsun (n/n), N.samsun (N/N), N. tabacum xamthi (nc), K326, N. tobacum White Burly, N. glutinosa, Nicotiano tabacum L. Nicotiana tabacum cv.Xanthi-nc, chenopodium amaranticolor, C. quinoa, .album, N.rustica, Peturnia hybrida, Lycopersicon escultentum., Nicandra physaioides as respectively. And all of the plant haven't any different with the blank. So all these dicotyledonous can't differentiate for MWEV and the host for MWEV.The leafhopper population were successfully constructed in the lab by maize and supplied bythe natural population and disease plant timing, under 25-28*C.the . bipunctella (mats) population were reared to 20 generation, 3000 per cultivated box. 5kg material were Gathered.The propagation of MEEV, transmit, entomological biology were guaranteed.3.The construction of C.bipunctella.(mats) of free virusCollecting from the field, separate from eggs, separate from ecdysis, separate through mixed leafhopper population were used to constructed the population of C.bipuncte!la.(mats) free from MWEV. In the field the C.bipunctella.(mats) was the precondition of the existing, occurring and spread of MWEV .and the MWEV indicating the existing of C.bipunctella.(mats). 24h after the C.bipunctella.(mats) was transfer to the maize, some of leafhopper can not transfer the virus.but it's offspring still have the ability to transmit MWEV, and almost same with the original population , All of the methods are unsuccessful. The population of C.bipunctella.(mats) collecting from the disease field were feed by maize, supply with the nature population or maize with MWEV timing. The experiment population that constructed by this method has similar behavior and transmit virus characteristic with the nature population.4.Effect of Temperature on of C bipunctella (mats)Development survivorship, longevity, and reproduction of Cicadulina bipunctella (mats) was measured at 15,20,25,28,32,35℃,.the population reared at 35℃ failed to develop. The population rear at 32℃,had the highest intrinsic rate of increase (0.0542),higher net reproduc...
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