Studies On Rapid Propagation In Salvia Mitiorrhiza, Pinellia Ternate And Virus-free In Pinellia Ternate

Plants are the important resources of natural medicament. Demand for natural medicinal materials exceeds supply. However, some natural medicinal plants are in short supply because of the destruction of natural environment and unplanted exploiting, It is an effective way to get medicament through manual culture, though with many problems such as poor quality and yield because of medicinal plants being infected by viruses, etc.Plant tissue culture technique was used for rapid propagation in Salvia mitiorrhiza, Pinellia ternate and virus-free in Pinellia ternate in our experiment We chose stem tip, stem ,leaves, petioles of Salvia mitiorrhiza and bulbil, leaves, petioles of Pinellia ternate for plant tissue culture. Rapid propagation and organogenesis were studied. The effects of phytohormones were studied on formation of callus, rapid propagation and organogenesis. Types and distribution of viruses in Pinellia ternate leaves were observed and identified preliminarily with electron microscope.The results of our experiment showed: (1)In MS medium with 1.0mg/L BA and 0.1mg/L NAA,the survival rate of stem tip in vitro was 91.4%. GA has a inhibited effect on the differentiation of stem tip. The effect of l.0mg/L BA for bud multiplication was the most effective, and 0.5mg/l IAA was beneficial for root-producing of tube plantlets. (2)A large number of plantlets were produced from bulbs on MS medium containing 2.0mg/L BA and 0.5mg/L GA. There were different states of morphogenesis when leaves of Pinellia ternate were cultured on MS medium containing different phytohormones. Roots was differentiated on MS medium containing 0.5mg/L IAA and plantlets was developed. The transplant survival rate of tube plantlets was about 98%. (3)Viruses which infected Pinellia ternate were preliminarily determined as spherical viruses, through being observed with electron microscope. Chroroplast in leaves of Pinellia ternate elapsed after infected by .viruses.(4)The treatment of high temperature and virus inhibiter was effective for virus-free of tube plantlets in Pinellia ternate.