| Pinus Massoniana, the main tree species in natural and artificial forests in China, especially in Southwest China, grows fast and adapts to variable conditions. The tree is used economically for multi-purposes. Pinus Massoniana seedlings are difficult to be survival, slow in growing and serious in soil born disease. The quality of the seedlings is necessary to be improved in artificial forestation.It was reported that the absorption of nutrients and water by seedlings could be promoted by inoculation of ectomycorrhizal fungi. The abilities of stress resistance, including drought and disease, could also be increased by this way, resulting the fast growing of seedlings. Inoculation of ectomycorrhizal fungi in nurse bed to produce ectomycorrhizal seedlings has been widely adopted abroad, but little in China, if any. It is necessary to point out that the inocunation of the ectomycorrhizal fungi suitable to local natural conditions is important in producing ectomycorhizal seedlings.In this experiment, typical ectomycorhizal fungi strains(Lactarius delicious 01, 02 and 03) were isolated from Three-Gorges Reservoir where Pinus Massoniana was very popular. And Suillus placidu 504(sp 504) and Laccaria bicolor S238N (Lb 23 8N) typical in South China were also chosen to study the effects of ectomycorrhizal fungi on the growth and nutrient absorption of Pinus Massoniana. And the culture characteristics of three strains of Lactarius delicious were also studied. The object of this study is to supply techniques in culturing ectomycorrhizal seedlings for planting trees in Three-Gorges Reservoir. The results are as follows:1. Growth of Lactarius deliciosus on PDA was better than that on Pachlewski. All selected tissues from different positions of the same Lactarius deliciosus basidiocarp can germinate mycelium on PDA. It showed that different position tissues could be used in isolation culture. According to the secretion of mycelium, Lactarius deliciosus strains were classified into Ld 01,Ld 02 and Ld 032. The isolated strains had better growth potential on PDA, and mycelium grew quickly and strongly. While they grew slowly and poorly on Pachlewski. It indicated that PDA was better in isolation and culturing Lactarius deliciosus than Pachlewski. The growth potentials of the three isolated strains were very different on PDA,Ld 01 was best,and no significant difference was found between Ld 02 and Ld 03.3. Better culture material components and conditions in preparing for inocula were as follows: 25-28℃ of temperature, PH value with 5.0-6.0, usage of PDA as culturing material, 65-70% of water content, solid material of perlite and vermiculite (V/V=1:1). The mycelium grew fast in flask and slowly in edible fungi planting sack and can. Generally, the time of solid material overgrowing with mycelium was about 25-40d, and the growth of mycelium of Lb S238N, Ld 01 and Ld 03 was fast, SP 504 was slow and Ld 02 was the worst.4.After inoculation with ectomycorrhizal, the root structure of infected seedlings was deformed, and no root hair was observed. Mycelium overgrew root tip. The infection rate was more than 65%, of which Lb S328N and Sp 504 were the highest, reaching 75%. Moreover, growth height, basal diameter and biomass of inoculated seedlings were also increased significantly. Lb S328N and Sp 504 increased the growth of seedlings more than three isolated Lactarius deliciosus strains. Compared with the control, the increments of height, basal diameter and biomass of seedlings of Lb S328N were 72.41%, 34.56% and 124.56%, respectively, and that of Sp 504 were 82.65%, 22.57% and 107.02%, respectively.5.The concentrations of N, P and K were significantly increased after inoculation. Compared with the control, the increments of N in underground and aboveground parts in inoculated seedlings were 17.83%-31.80% and 11.17%-26.08%( P<0.05), those of P were 32.11%-44.46% and 26.23%-40.79%( P<0.01), and those of K were 15.81%-38.66% and 12.62%-23.33%.In conclusion, ectomycorrhizal fungus inoculation can increase nut...
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