Effect Of Cysteamine On The Somatostatin Cell And Mucosal Immunization In The Intestine Of Chicken

1. Eighty-four 1-day-old cock were divided into control group, ND treated group, CS treated group and the group was immunized with Newcastle disease attenuated vaccine by digestive rout after feeding cysteamine(CN group). The cocks were first immunized on 8-day-old and were killed on 21, 35 and 49 -day-old. The distribution and change of somatostatin(SS)-positive cells, IgA-positive cells, T lymphocytes and mast cells in duodenum and jejunum of chickens were studied.The distribution and change of SS-positive cells were studied with immunohistochemical technique. The results showed the SS-positive cells of the small intestinal mainly distributed around intestinal gland and between the epithelial cells of small intestine. The number of SS-positive cells in duodenum of ND treating group increased significantly (P<0.Ol) compared to the control group at 3 weeks after oral immunization. At 5 weeks, the number of SS-positive cells in jejunum of ND treated group increased significantly (P<0.05) compared to the control group, The number of SS-positive cells in duodenum and jejunum of CS treated group increased significantly (P<0.01) compared to the control group, the number of SS-positive cells in duodenum of CN group increased significantly (P<0.01) compared to the control group and the SS-positive cells in jejunum increased significantly(P<0.05) compared to the control group. The results suggest that the cytokine produced by lymphocytes after mucosal immunization can stimulate the production of SS-positive cells and prove that mucosal immunization can influence the neuroendocrinology system in the chicken.The change of the number of IgA-positive cells and Acid-naphthy-1 acetate esterase (ANAE)-positive cells in chicken small intestinal were studied with immunohistochemical technique and histology method. The results showed that the number of IgA-positive cells in duodenum of CN group increased significantly (P<0.01) compared to the group immunizing only with Newcastle disease attenuated vaccine at 3 week after oral immunization; Comparing to ND treating group, control group and CS treating group, the number of IgA-positive cells in jejunum increased significantly (P<0.01). At 5 week, thenumber of IgA-positive cells in duodenum of CN group increased significantly (P<0.01) compared to the group immunizing only with Newcastle disease attenuated vaccine; the number of IgA-positive cells in jejunum increased significantly (P<0.01) than the control group. At 7 week, the number of ANAE-positive cells in duodenum of CN group increased significantly (P<0.01) than the control group. The results suggested young chickens were immunized with Newcastle disease attenuated vaccine by digestive rout after feed cysteamine to decrease the level of somatostatin, the number of IgA-positive cells and ANAE-positive cells increased, thus mucosal immunization was promoted.Toluidine blue staining method was used to study the change of r mast cells numble in chicken small intestinal. The results showed that the number of mast cells in duodenum of CN group increased significantly (P<0.01) compared to the group immunizing only with Newcastle disease attenuated vaccine at 3 week after oral immunization; Comparing to the control group at 5 week, the number of mast cells among experiment group increased significantly, though no differences existed among all groups. Our results suggest the number of mast cells might reflect the digestive mucosal immunization. 2. Broilers were immunized with Newcastle disease attenuated vaccine by digestive rout after feeding two doses of cysteamine (20mg/kg?CS-I group; 80mg/kg ?CS-II group). The content of IL-2 in spleen and duodenum was tested by RIA. The results showed the content of IL-2 in spleen and duodenum of CS-I group increased significantly (P<0.05) compared to the group immunizing only Newcastle disease attenuated vaccine at 3 week after oral immunization; the content of IL-2 in duodenum of CS-II group increased significantly (P<0.01) compared to the group immunizing only with Newcastle disease atten...