| Kang 201S,newly breeded by Rice Research Institute of Hunan Agricultural University,is a photo-thermo sensitive genie male sterile line.And the hybrid new combination Kang 201/ R527, combinated with Kang 201S and Chuanhui 527,shows higher yield,good grain qulity and stronger resistance in the regional experiments of Hunan Province,and will be popularized very soon.The applied prospect of the combination is good. The charateristics of the combination, including temperature and photoperiod sensitivity, outcrossing traits,and corresponding techniques of seed production were studied and discussed in this dissertation.The main results were as follows:1 R527,whose sensitive level of photoperiod and temperature was first and third respectively, was classfied as weak photoperiod and temperature sensitive variety.However,Kang 201S, whose sensitive level of photoperiod and temperature was seventh and eighth respectively,belonged to strong photoperiod and temperature sensitive variety.And the sensitivity to photoperiod and temperature between them were different.2 The days to initial heading of R527,which was 101 to 73 days by split seeding from March ,30 to July,10,had a significant linear relation with EAT in different growth stage,and had a extremely significant linear relation with EAT from seeding to 3-leaf stage.The C.V (Coefficient of Variation) of EAT from seeding to 6-leaf stage (transplanting stage) was much bigger(>10%),and the same one from 6-leaf stage to initial heading was rather smaller.The days to initial heading of Kang 201S,which was 95 to 55 days in different seeding dates,didn't show the significant linear relation with EAT in different growth stage.The C.V of EAT from seeding to initial heading was rather bigger(>10%).And EAT of R527 during the whole days to initial heading was more stable than the same one of Kang 201S.3 The heading stage of R527 was about 8 days.and the heading characteristic was relatively accumulative.However,the heading stage of Kang 201S was about 15 days,and the heading characteristic wasn't uniform.Compared with R527,although the flowering stage of single panicle of Kang 201S wasn't significantly different,the flowering stage of single cave was obviously longer.The synchronization level of flowering stage between parents was low.And the whole synchronization rate of anthersis wasn't high under either high temperature or normal temperature condition(33℃ and 27.4℃).4 The pollen amount of R527,whose pollens in per anther was about 1199,was superior to R80,Minghui 77,R288 and Minghui 63,and equal to R207.The stigma exsertion rate of Kang 201S was lower than that of Peiai 64S,higher than that of Lu 18S.The spikelet opening angle of Kang 201S was much bigger than that of Peiai 64S or Lu 18S,but the stigma bulk of Kang 201s was smaller than the latter ones.5 By daily 6-hour treatment to R527 in differentiating 5th stage under mean high temperature of 36.33℃ condition,the result of 3-day treatment wasn't significant.And 6-hour treatment showed that the amount of normal pollons reduced,pollen activity got weaker, incrossing seed set rate decreased. Moreover,the longer the treatment time was,the worse the harm was. By daily continous treatment to R527 in differentiating 7th stage under mean low temperature of 20℃,the influence of 3-day or 6-day treatment wasn't significant.If the treatment time got longer,the harm existed.And the harmful performance of low temperature showed that the amount of pollens reduced,pollen activity got weaker,the amount of degenerative spikelets increased,incrossing seed set rate decreased,and the days to initial heading became longer.High temperature harm was worse than low temperature one.6 The trends of POD activity in leaves of R527,which were different under between high temperature and low temperature condition,were both relative to the change of pollen fertility.Under high temperature condition,the contents of starch,praline and soluble protein in anther reduced,and t... |
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